Creating rat model for hypoxic brain damage in neonates by oxygen deprivation.
Creating rat model for hypoxic brain damage in neonates by oxygen deprivation.
复制标题
创建新生儿缺氧脑损伤大鼠模型
DOI：
10.1371/journal.pone.0083589
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Cui H
中科院分区：
文献类型：
--
作者：
Zhang Q;Ding Y;Yao Y;Yu Y;Yang L;Cui H
本研究探讨了采用非手术缺氧方法建立新生大鼠缺氧性脑损伤模型的可行性。将7日龄SD大鼠置于低氧（8%）环境中1.5h。第二组在1.5小时低氧治疗前结扎双侧头动脉，方法类似于流行的Rice方法，使大脑暴露于缺氧和缺血的情况。低氧治疗后1天，评价短期神经功能和脑水重量。在缺氧处理后第1天和第3天进行脑病理学和组织学检查。与对照组相比，两组均显示出神经功能受损和脑水重量增加。组织学研究还显示，两组大脑皮层下、海马和侧脑室都有损伤。两组脑损伤程度无显著性差异。我们的工作表明，缺氧本身就足以引起类似于缺氧缺血性脑疾病（HIBD）的脑损伤。由于这种方法避免了创伤性手术操作，因此减少了实验动物在实验过程中的压力和死亡率，我们建议它是建立HIBD研究大鼠模型的有利方法。
Current study explores the feasibility of using a non-surgical method of oxygen deprivation to create Hypoxic brain damage in neonatal rats for medical studies. 7-day-old Sprague Dowley (SD) rats were kept in a container with low oxygen level (8%) for 1.5h. A second group had bilateral cephalic artery ligation before the 1.5h-low oxygen treatment, a method similar to the popular Rice method, to expose the brain to both hypoxic and ischemic situations. Short term neural functions and brain water weights were evaluated 1 day after the hypoxic treatment. Brain pathology and histology were also examined at 1 day and 3 days after the hypoxic treatment. Both groups showed impaired neural functions and increased brain water weight compared to the controls. Histology studies also revealed injuries in the subcortex, hippocampus and lateral ventricle in the brains from both groups. There is no significant difference in the degree of brain damages observed in the two groups. Our work demonstrated that oxygen deprivation alone is sufficient to cause brain damages similar to those seen in Hypoxic-ischemic brain disease (HIBD). Because this method avoids the invasive surgical procedure and therefore reduces the stress and mortality of laboratory animals during the experiment, we recommend it to be the favorable method for creating rat models for HIBD studies.
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