An immunohistochemical study of the antinociceptive effect of calcitonin in ovariectomized rats.
An immunohistochemical study of the antinociceptive effect of calcitonin in ovariectomized rats.
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DOI：
10.1186/1471-2474-9-164
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发表时间：
2008-12-15
影响因子：
2.3
通讯作者：
Sekiguchi M
中科院分区：
文献类型：
--
作者：
Takayama B;Kikuchi S;Konno S;Sekiguchi M
降钙素用于治疗高钙血症时降低血钙浓度，以及改善骨质疏松症时的骨量。在临床情况中已观察到并报道了降钙素的镇痛作用。卵巢切除（OVX）大鼠表现出与患有骨质疏松症的人类中观察到的相同的激素变化，并且是绝经后骨质疏松症的动物模型。本研究的目的是通过免疫组织化学研究探讨降钙素对 OVX 大鼠的抗伤害作用。我们使用免疫组织化学方法评估了降钙素在卵巢切除（OVX）大鼠模型中的抗伤害作用，该模型表现出骨质疏松和痛觉过敏。 15 只大鼠进行双侧卵巢切除，10 只大鼠接受与卵巢切除术相同的手术作为假模型。我们使用了五组：OVX-CT (n = 5)、sham-CT (n = 5) 和 OVX-CT-pcpa (n = 5) 组接受降钙素（CT：4 U/kg/天），而 OVX-vehi (n = 5) 和 sham-vehi (n = 5) 组每周皮下注射 5 次，持续 4 周。 OVX-CT-pcpa组在降钙素注射的最后3天腹腔注射对氯苯丙氨酸（pcpa；血清素生物合成抑制剂）（100 mg/kg/天）。将 5% 福尔马林 (0.05 ml) 皮下注入后爪两小时后，取出 L5 脊髓，并使用 Mann-Whitney-U 测试评估 Fos 免疫反应性 (ir) 神经元的数量。 OVX-CT 和假 CT 组中 Fos-ir 神经元的数量分别显着少于 OVX-vehi 和 sham-vehi 组 (p = 0.0090, p = 0.0090)。 OVX-CT-pcpa 组中 Fos-ir 神经元的数量显着多于 OVX-CT 组 (p = 0.0283)，这意味着 pcpa 抑制降钙素诱导的 c-Fos 产生减少。本研究结果表明：1）c-Fos 的增加可能与 OVX 大鼠的痛觉过敏有关。 2) 降钙素对 OVX 和假大鼠均具有抗伤害作用。 3) 中枢血清素能系统参与降钙素的抗伤害特性。
Calcitonin is used as a treatment to reduce the blood calcium concentration in hypercalcemia and to improve bone mass in osteoporosis. An analgesic effect of calcitonin has been observed and reported in clinical situations. Ovariectomaized (OVX) rats exhibit the same hormonal changes as observed in humans with osteoporosis and are an animal model of postmenopousal osteoporosis. The aim of this study to investigate antinociceptive effect of calcitonin in OVX rats using the immunohistochemical study. We assessed the antinociceptive effects of calcitonin in an ovariectomized (OVX) rat model, which exhibit osteoporosis and hyperalgesia, using the immunohistochemical method. Fifteen rats were ovariectomized bilaterally, and ten rats were received the same surgery expected for ovariectomy as a sham model. We used five groups: the OVX-CT (n = 5), the sham-CT (n = 5), and the OVX-CT-pcpa (n = 5) groups recieved calcitonin (CT: 4 U/kg/day), while OVX-vehi (n = 5) and the sham-vehi (n = 5) groups received vehicle subcutaneously 5 times a week for 4 weeks. The OVX-CT-pcpa-group was given traperitoneal injection of p-chlorophenylalanine (pcpa; an inhibitor of serotonin biosynthesis) (100 mg/kg/day) in the last 3 days of calcitonon injection. Two hours after 5% formalin (0.05 ml) subcutaneously into the hind paw, the L5 spinal cord were removed and the number of Fos-immunoreactive (ir) neurons were evaluated using the Mann-Whitney-U test. The numbers of Fos-ir neurons in the OVX-CT and sham-CT groups were significantly less than in the OVX-vehi and sham-vehi groups, respectively (p = 0.0090, p = 0.0090). The number of Fos-ir neurons in the OVX-CT-pcpa-group was significantly more than that of the OVX-CT-group (p = 0.0283), which means pcpa inhibits calcitonin induced reduction of c-Fos production. The results in this study demonstrated that 1) the increase of c-Fos might be related to hyperalgesia in OVX-rats. 2) Calcitonin has an antinociceptive effect in both OVX and sham rats. 3) The central serotonergic system is involved in the antinociceptive properties of calcitonin.
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影响因子：
6.8
作者：
Karsdal, Morten A.;Henriksen, Kim;Christiansen, Claus
通讯作者：
Christiansen, Claus
影响因子：
7.4
作者：
ELLIOTT, KJ;BRODSKY, M;INTURRISI, CE
通讯作者：
INTURRISI, CE
影响因子：
2.9
作者：
GUIDOBONO, F;NETTI, C;FERRI, S
通讯作者：
FERRI, S
影响因子：
2.5
作者：
MENETREY, D;GANNON, A;BASBAUM, AI
通讯作者：
BASBAUM, AI
影响因子：
2.9
作者：
FABBRI, A;FRAIOLI, F;PERT, A
通讯作者：
PERT, A