Host- plasmid network structure in wastewater is linked to antimicrobial resistance genes.
Host- plasmid network structure in wastewater is linked to antimicrobial resistance genes.
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DOI：
10.1038/s41467-024-44827-w
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发表时间：
2024-01-16
影响因子：
16.6
通讯作者：
Sanders, Dirk
中科院分区：
文献类型：
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作者：
Risely, Alice;Newbury, Arthur;Stalder, Thibault;Simmons, Benno I.;Top, Eva M.;Buckling, Angus;Sanders, Dirk
作为移动遗传元件，质粒对于我们了解微生物群落中抗菌素耐药性的传播至关重要。质粒对其宿主细菌具有不同的适应性影响，这将显着影响它们作为抗菌素耐药性载体的作用。使用质粒群体模型，我们首先表明，当嵌入具有多个宿主和质粒的群落时，有益的质粒比昂贵的质粒与更多数量的宿主相互作用。然后，我们从 Hi-C 宏基因组数据集中分析了天然宿主-质粒废水群落的网络。正如模型预测的那样，我们发现抗菌素耐药性编码质粒可能对其废水中的宿主产生积极的适应性影响，与非抗菌素耐药性质粒相比，与更多的细菌类群相互作用，并且与非抗菌素耐药性质粒相比，它们对于连接整个网络的重要性不成比例。这凸显了抗菌药物通过增加携带抗菌素耐药性的质粒的益处而在重组宿主质粒网络中的作用，这可能对抗菌素耐药性基因通过微生物网络的传播产生影响。此外，抗菌素耐药性编码质粒与更广泛的宿主相关，这意味着它们对细菌菌株的更新更加稳健。作者将理论和微生物生态学模型应用于废水样本，结果表明，与不携带耐药基因的质粒相比，携带抗菌药物耐药性的质粒与更多、更多样化的细菌相互作用。
As mobile genetic elements, plasmids are central for our understanding of antimicrobial resistance spread in microbial communities. Plasmids can have varying fitness effects on their host bacteria, which will markedly impact their role as antimicrobial resistance vectors. Using a plasmid population model, we first show that beneficial plasmids interact with a higher number of hosts than costly plasmids when embedded in a community with multiple hosts and plasmids. We then analyse the network of a natural host-plasmid wastewater community from a Hi-C metagenomics dataset. As predicted by the model, we find that antimicrobial resistance encoding plasmids, which are likely to have positive fitness effects on their hosts in wastewater, interact with more bacterial taxa than non-antimicrobial resistance plasmids and are disproportionally important for connecting the entire network compared to non- antimicrobial resistance plasmids. This highlights the role of antimicrobials in restructuring host-plasmid networks by increasing the benefits of antimicrobial resistance carrying plasmids, which can have consequences for the spread of antimicrobial resistance genes through microbial networks. Furthermore, that antimicrobial resistance encoding plasmids are associated with a broader range of hosts implies that they will be more robust to turnover of bacterial strains. Authors apply theory and microbial ecology modelling to a wastewater sample, and show that antimicrobial resistance carrying plasmids interact with a higher number and more diverse range of bacteria than plasmids that do not carry resistance genes.
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DOI：
10.1038/ismej.2012.135
发表时间：
2013-03
期刊：
The ISME journal
影响因子：
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作者：
通讯作者：
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影响因子：
3.9
作者：
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DOI：
10.1098/rspb.2009.0635
发表时间：
2009-08-22
影响因子：
4.7
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通讯作者：
Dajoz, Isabelle
DOI：
10.1073/pnas.1600974113
发表时间：
2016-07-19
影响因子：
11.1
作者：
Hall, James P. J.;Wood, A. Jamie;Brockhurst, Michael A.
通讯作者：
Brockhurst, Michael A.
影响因子：
5.2
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