UBE2C Is a Potential Biomarker of Intestinal-Type Gastric Cancer With Chromosomal Instability.
UBE2C Is a Potential Biomarker of Intestinal-Type Gastric Cancer With Chromosomal Instability.
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UBE2C 是染色体不稳定的肠型胃癌的潜在生物标志物
DOI：
10.3389/fphar.2018.00847
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发表时间：
2018
影响因子：
5.6
通讯作者：
Yu Y
中科院分区：
文献类型：
--
作者：
Zhang J;Liu X;Yu G;Liu L;Wang J;Chen X;Bian Y;Ji Y;Zhou X;Chen Y;Ji J;Xiang Z;Guo L;Fang J;Sun Y;Cao H;Zhu Z;Yu Y
This study explored potential biomarkers associated with Lauren classification of gastric cancer. We screened microarray datasets on gastric cancer with information of Lauren classification in gene expression omnibus (GEO) database, and compared differentially expressing genes between intestinal-type or diffuse-type gastric cancer. Four sets of microarray data (GSE2669, GSE2680, GDS3438, and GDS4007) were enrolled into analysis. By differential gene analysis, UBE2C, CDH1, CENPF, ERO1L, SCD, SOX9, CKS1B, SPP1, MMP11, and ANLN were identified as the top genes related to intestinal-type gastric cancer, and MGP, FXYD1, FAT4, SIPA1L2, MUC5AC, MMP15, RAB23, FBLN1, ANXA10, and ADH1B were genes related to diffuse-type gastric cancer. We comprehensively validated the biological functions of the intestinal-type gastric cancer related gene UBE2C and evaluated its clinical significance on 1,868 cases of gastric cancer tissues from multiple medical centers of Shanghai, China. The gain of copy number on 20q was found in 4 out of 5 intestinal-type cancer cell lines, and no similar copy number variation (CNV) was found in any diffuse-type cancer cell line. Interfering UBE2C expression inhibited cell proliferation, migration and invasion in vitro, and tumorigenesis in vivo. Knockdown of UBE2C resulted in G2/M blockage in intestinal-type gastric cancer cells. Overexpression of UBE2C activated ERK signal pathway and promoted cancer cell proliferation. U0126, an inhibitor of ERK signaling pathway reversed the oncogenic phenotypes caused by UBE2C. Moreover, overexpression of UBE2C was identified in human intestinal-type gastric cancer. Overexpression of UBE2C protein predicted poor clinical outcome. Taken together, we characterized a group of Lauren classification-associated biomarkers, and clarified biological functions of UBE2C, an intestinal-type gastric cancer associated gene. Overexpression of UBE2C resulted in chromosomal instability that disturbed cell cycle and led to poor prognosis of intestinal-type gastric cancer.
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泛素结合酶 2C (UBE2C) 高表达与鼻咽癌进展相关
影响因子：
3.8
作者：
Shen Z;Jiang X;Zeng C;Zheng S;Luo B;Zeng Y;Ding R;Jiang H;He Q;Guo J;Jie W
通讯作者：
Jie W
影响因子：
7.5
作者：
Li, Linwei;Li, Xiaoyan;Shi, Zuxuan
通讯作者：
Shi, Zuxuan
影响因子：
4.6
作者：
Cacciola, Nunzio Antonio;Calabrese, Chiara;Pallante, Pierlorenzo
通讯作者：
Pallante, Pierlorenzo
胃癌组织病理学分类与预后的队列研究及荟萃分析
影响因子：
2.8
作者：
Liu, Lei;Wang, Zhi W.;Yu, Ying Y.
通讯作者：
Yu, Ying Y.
DOI：
10.1111/apm.1965.64.1.31
发表时间：
1965-01-01
期刊：
ACTA PATHOLOGICA ET MICROBIOLOGICA SCANDINAVICA
影响因子：
--
作者：
LAUREN, P
通讯作者：
LAUREN, P