Vasorelaxant Properties of n-3 Polyunsaturated Fatty Acids in Aortas from Spontaneously Hypertensive and Normotensive Rats
Vasorelaxant Properties of n-3 Polyunsaturated Fatty Acids in Aortas from Spontaneously Hypertensive and Normotensive Rats
复制标题
自发性高血压和血压正常大鼠主动脉中 n-3 多不饱和脂肪酸的血管舒张特性
DOI：
10.1177/174182679400100112
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发表时间：
1994
影响因子：
--
通讯作者：
P. Ursell
中科院分区：
文献类型：
--
作者：
M. Engler;M. Engler;P. Ursell
背景：动物研究和临床试验均显示，饮食中食用富含 n-3 多不饱和脂肪酸、二十二碳六烯酸 (DHA) 和二十碳五烯酸 (EPA) 的鱼可以降低血压。尽管抗高血压机制尚不清楚，但 n-3 多不饱和脂肪酸的降血压作用可能部分归因于其血管舒张特性。方法：将 16-17 周龄 Wistar-Kyoto (WKY) 和自发性高血压大鼠 (SHR) 的有内皮和无内皮的主动脉环悬浮在组织浴中并测量等长张力。生成去甲肾上腺素收缩环中 DHA 和 EPA (1-100 μmol/l) 的浓度响应曲线。使用尾套法测量血压并测定主动脉中膜厚度。结果：与 WKY（n = 10；124 ± 1.2 mmHg，P ≤ 0.0001）相比，SHR（n = 10；194 ± 4.4 mmHg）的血压显着升高。 DHA (1–100μmol/l) 以浓度依赖性方式松弛 WKY（-3.3 ± 0.7 至 -13 ± 2.3%，P ≤ 0.001）和 SHR（-6.5 ± 1.8 至 -22.9 ± 4%，P ≤ 0.01）的主动脉环。 EPA（1–100μmol/l）在SHR（−10.1±2.0至-33±3.9％，P <0.01）中引起比WKY（−2.9±1.1至−18.3±2.1％，P <0.01）主动脉环更大的松弛。 DHA 在 WKY 和 SHR 中的松弛作用以及 EPA 在 WKY 中的松弛作用不依赖于完整的内皮。然而，EPA (1–10μmol/l) 在完整的 SHR 环中诱导的反应（−10.1 ± 2.0 至 -14.5 ± 3.1%）比去内皮化的 SHR 环（0 至 -2.1 ± 1.7%，P = 0.001）更大。结论：在 WKY 和 SHR 中观察到的 n-3 脂肪酸的直接松弛作用可能在一定程度上有助于膳食鱼和鱼油补充剂的降血压作用。
Background: Dietary consumption of fish, rich in n-3 polyunsaturated fatty acids, docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA), has been shown to reduce blood pressure in both animal studies and clinical trials. Although the antihypertensive mechanisms are not known, the blood-pressure-lowering effect of n-3 polyunsaturated fatty acids may be partially attributed to their vasorelaxant properties. Methods: Aortic rings, with and without endothelium, from Wistar-Kyoto (WKY) and spontaneously hypertensive rats (SHR), 16–17 weeks old, were suspended in tissue baths and isometric tension was measured. Concentration-response curves were generated for DHA and EPA (1–100 μmol/l) in norepinephrine-contracted rings. Blood pressure was measured using the tail-cuff method and aortic media thickness was determined. Results: Blood pressure was significantly increased in SHR (n = 10; 194 ± 4.4 mmHg) compared with WKY (n = 10; 124 ± 1.2 mmHg, P ≤ 0.0001). DHA (1–100μmol/l) relaxed aortic rings from WKY (-3.3 ± 0.7 to −13 ± 2.3%, P ≤ 0.001) and from SHR (-6.5 ± 1.8 to −22.9 ± 4%, P ≤ 0.01) in a concentration-dependent manner. EPA (1–100μmol/l) evoked greater relaxation in SHR (−10.1 ± 2.0 to-33 ± 3.9%, P <0.01) than in WKY (−2.9 ± 1.1 to −18.3 ± 2.1%, P <0.01) aortic rings. The relaxant effect of DHA in both WKY and SHR and of EPA in WKY were not dependent on an intact endothelium. However, EPA (1–10μmol/l) induced greater responses in intact SHR rings (−10.1 ± 2.0 to −14.5 ± 3.1%) than in de-endothelialized SHR rings (0 to −2.1 ± 1.7%, P = 0.001). Conclusion: The direct relaxant effects of n-3 fatty acids as seen in WKY and SHR may contribute, in part, toward the blood-pressure-lowering effect of dietary fish and fish-oil supplementation.
膳食鱼油对易发生中风的自发性高血压大鼠主动脉 Rb 流出的影响。
影响因子：
3.2
作者：
Smith,JM;Paulson,DJ;Labak,S
通讯作者：
Labak,S
影响因子：
8.3
作者：
BOULANGER, CM;VANHOUTTE, PM
通讯作者：
VANHOUTTE, PM