Effects of Heat Stress on Production Performance, Redox Status, Intestinal Morphology and Barrier-Related Gene Expression, Cecal Microbiome, and Metabolome in Indigenous Broiler Chickens.
Effects of Heat Stress on Production Performance, Redox Status, Intestinal Morphology and Barrier-Related Gene Expression, Cecal Microbiome, and Metabolome in Indigenous Broiler Chickens.
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热应激对本地肉鸡生产性能、氧化还原状态、肠道形态和屏障相关基因表达、盲肠微生物组和代谢物组的影响。
DOI：
10.3389/fphys.2022.890520
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发表时间：
2022
影响因子：
4
通讯作者：
Jha, Rajesh
中科院分区：
文献类型：
--
作者：
Liu, Wen-Chao;Pan, Zi-Yi;Zhao, Yue;Guo, Yan;Qiu, Sheng-Jian;Balasubramanian, Balamuralikrishnan;Jha, Rajesh
关键词：
本研究旨在探讨热应激对本地肉鸡生产性能、氧化还原状态、小肠屏障相关参数、盲肠菌群和代谢物组的影响。选用56日龄本地肉仔鸡40只，随机分为正常对照组（NT组，21.3 ± 1.2 ℃，24 h/d）和高温对照组（HS组，32.5 ± 1.4 ℃，8 h/d），每组5个重复，进行为期4周的饲养试验。结果表明，在3-4周龄和1-4周龄，HS组肉鸡增重显著低于NT组（p < 0.05）; HS暴露使腹脂率增加（p < 0.05），但使股肌率降低（p < 0.01）。此外，HS组胸肌滴水损失显著高于NT组（p < 0.01）。结果表明，HS组血清和空肠总抗氧化能力（T-AOC）、空肠总超氧化物歧化酶（T-SOD）活性、大腿肌、十二指肠和空肠谷胱甘肽过氧化物酶（GSH-Px）活性以及胸肌、十二指肠和空肠过氧化氢酶（CAT）活性均显著降低（p < 0.05）。而胸肌、十二指肠和空肠中的丙二醛（MDA）含量显著升高（p < 0.05）。此外，HS暴露还下调了十二指肠中Occludin和ZO-1，空肠中Occludin、Claudin-1、Claudin-4、ZO-1、Mucin-2以及回肠中Claudin-4和Mucin-2的相对mRNA表达（p < 0.05）。16 SrRNA测序结果显示，在属水平上，HS组盲肠中厌氧食虫菌的相对丰度增加（p < 0.05）。盲肠代谢组学分析表明两组之间有19种差异代谢物（p < 0.10，VIP >1）。京都基因和基因组百科全书（KEGG）分析显示，差异代谢物主要富集在柠檬酸循环（TCA循环）等10个信号通路中（p < 0.01）。综上所述，慢性HS暴露导致本地肉鸡生产性能下降，抗氧化能力降低，肠道屏障功能破坏，盲肠微生物群和代谢组产生负面影响。
This study was done to evaluate the effects of heat stress (HS) on production performance, redox status, small intestinal barrier-related parameters, cecal microbiota, and metabolome of indigenous broilers. A total of forty female indigenous broilers (56-day-old) were randomly and equally divided into normal treatment group (NT group, 21.3 ± 1.2°C, 24 h/day) and HS group (32.5 ± 1.4°C, 8 h/day) with five replicates of each for 4 weeks feeding trial. The results showed that the body weight gain (BWG) of broilers in HS group was lower than those in NT group during 3–4 weeks and 1–4 weeks (p < 0.05). The HS exposure increased the abdominal fat rate (p < 0.05) but decreased the thigh muscle rate (p < 0.01). Besides, broilers in HS group had higher drip loss of breast muscle than NT group (p < 0.01). Broilers exposed to HS had lower total antioxidant capacity (T-AOC) in serum and jejunum, activities of total superoxide dismutase (T-SOD) in the jejunum, glutathione peroxidase (GSH-Px) in the thigh muscle, duodenum, and jejunum; and catalase (CAT) in breast muscle, duodenum, and jejunum (p < 0.05). Whereas the malondialdehyde (MDA) contents in breast muscle, duodenum, and jejunum was elevated by HS exposure (p < 0.05). Moreover, the relative mRNA expression of Occludin and ZO-1 in the duodenum, Occludin, Claudin-1, Claudin-4, ZO-1, Mucin-2 in the jejunum, and the Claudin-4 and Mucin-2 in the ileum was down-regulated by HS exposure (p < 0.05). The 16S rRNA sequencing results showed that the HS group increased the relative abundance of Anaerovorax in the cecum at the genus level (p < 0.05). Cecal metabolomics analysis indicated 19 differential metabolites between the two groups (p < 0.10, VIP >1). The Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed that the differential metabolites mainly enriched in 10 signaling pathways such as the Citrate cycle (TCA cycle) (p < 0.01). In summary, chronic HS exposure caused a decline of production performance, reduced antioxidant capacity, disrupted intestinal barrier function, and negatively affected cecal microbiota and metabolome in indigenous broilers.
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影响因子：
4.4
作者：
Berri, C.;Besnard, J.;Relandeau, C.
通讯作者：
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影响因子：
1.7
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Guo Y;Liao JH;Liang ZL;Balasubramanian B;Liu WC
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Liu WC
影响因子：
3
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An, Lilong
影响因子：
3
作者：
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通讯作者：
Izuddin, Wan Ibrahim
影响因子：
2.7
作者：
Cheng, Yefei;Du, Mingfang;Zhou, Yanmin
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