Hepatic lipid metabolomics in response to heat stress in local broiler chickens breed (Huaixiang chickens).
Hepatic lipid metabolomics in response to heat stress in local broiler chickens breed (Huaixiang chickens).
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DOI：
10.1002/vms3.462
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发表时间：
2021-07
影响因子：
1.7
通讯作者：
Liu WC
中科院分区：
文献类型：
--
作者：
Guo Y;Liao JH;Liang ZL;Balasubramanian B;Liu WC
高温环境诱发的热应激（HS）是一种对动物有害的环境因素，可导致动物生理和代谢功能发生剧烈变化。然而，缓慢生长肉鸡肝脏脂质代谢的代谢组学水平机制仍不清楚。本研究研究了HS对中国本土慢生长肉鸡（淮乡鸡）肝脏脂质组学的影响。试验分为2个处理，每个处理5个重复，每笼4只鸡，选取平均初始体重为840.75±20.79 g的8周龄怀香鸡母鸡40只，随机分为常温组（NT）和高温组（HS），温度分别为21.3±1.2℃和32.5±1.4℃，试验期为4周。两组相对湿度保持在55% ~ 70%。采用液相色谱-质谱（LC - MS）代谢组学方法评价肉鸡肝脏脂质组学的变化。结果表明，两种处理之间存在12种差异代谢物。与NT组相比，HS组肝脏磷脂酰胆碱（PC）（16:0/16:0）、磷脂酰胆碱（PC）（16:0/16:0）、甘油三酯（TG）（16:0/16:1/18:1）、甘油三酯（TG）（18:0/18:1）、甘油三酯（TG）（18:0/18:1）、甘油三酯（TG） （VIP >）水平降低（p < 0.05）， PC（18:0/22:5）、二甲基-磷脂酰乙醇胺（dMePE）（14:0/18:3）、dMePE（18:0/18:1）、dMePE（16:0/20:3）水平升高（投影变量重要性；VIP >）， p < 0.05)。此外，从代谢途径分析可知，亚麻酸、α -亚麻酸、甘油脂和甘油磷脂代谢途径参与了HS对肉仔鸡肝脏脂质代谢的影响（p < 0.05）。综上所述，HS主要通过亚油酸、α -亚麻酸、甘油脂和甘油磷脂代谢途径改变了土产肉鸡肝脏脂质代谢。这些发现为研究HS对中国土生肉鸡肝脏脂质组学的影响提供了新的思路。本研究采用液相色谱-质谱联用技术（LC - MS）研究了热应激对中国本土慢生长肉鸡（怀香鸡）肝脏脂质组学的影响。结果表明，热应激改变了肉仔鸡肝脏14种脂质代谢产物的水平，并改变了4条脂质代谢途径。这些发现扩大了热应激对中国本土肉鸡肝脏脂质组学影响的认识。
High‐temperature environment‐induced heat stress (HS) is a hazard environmental element for animals, leading to dramatic changes in physiological and metabolic function. However, the metabolomic‐level mechanisms underlying lipid metabolism in liver of slow‐growing broilers are still obscure. The present study investigated the effects of HS on hepatic lipidomics in Chinese indigenous slow‐growing broilers (Huaixiang chickens). The study includes two treatments, each treatment had 5 replicates with 4 broilers per cage, where a total of 40 eight‐week‐old female Huaixiang chickens (average initial body weight of 840.75 ± 20.79 g) were randomly divided into normal temperature (NT) and HS groups for 4 weeks, and the broilers of NT and HS groups were exposed to 21.3 ± 1.2℃ and 32.5 ± 1.4℃ respectively. The relative humidity of the two groups was maintained at 55%–70%. The liquid chromatography‐mass spectrometry (LC‐MS)‐based metabolomics were conducted to evaluate the changes in hepatic lipidomics of broilers. The results showed that there were 12 differential metabolites between the two treatments. Compared with the NT group, HS group reduced the levers of hepatic phosphatidylcholine (PC) (16:0/16:0), PC (16:0/18:2), triglyceride (TG) (16:0/16:1/18:1), TG (18:0/18:1/20:4) (VIP > 1 and p < 0.05), while increased PC (18:1/20:3), PC (18:0/18:1), PC (18:1/18:1), PC (18:0/22:5), dimethyl‐phosphatidyl ethanolamine (dMePE) (14:0/18:3), dMePE (18:0/18:1) and dMePE (16:0/20:3) levels (Variable Importance in the Projection; VIP > 1 and p < 0.05). In addition, according to the analysis of metabolic pathway, the pathways of linoleic acid, alpha‐linolenic acid, glycerolipid and glycerophospholipid metabolism were involved in the effects of HS on hepatic lipid metabolism of broilers (p < 0.05). In conclusion, HS altered the hepatic lipid metabolism mainly through linoleic acid, alpha‐linolenic acid, glycerolipid and glycerophospholipid metabolism pathway in indigenous broilers. These findings provided novel insights into the role of HS on hepatic lipidomics in Chinese indigenous broiler chickens. The present study was conducted to investigate the effects of heat stress on hepatic lipidomics in Chinese indigenous slow‐growing broiler chickens (Huaixiang chickens) through using liquid chromatography‐mass spectrometry (LC‐MS). The results demonstrated that heat stress changed the levels of 14 hepatic lipid metabolites, and altered four lipid metabolic pathways in broilers. These findings expanded understandings of the effects of heat stress on hepatic lipidomics in Chinese indigenous broiler chickens.
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影响因子：
4.4
作者：
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通讯作者：
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影响因子：
1.7
作者：
Guo Y;Liao JH;Liang ZL;Balasubramanian B;Liu WC
通讯作者：
Liu WC
影响因子：
4
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