Approaches to Interchain Cysteine-Linked ADC Characterization by Mass Spectrometry.
Approaches to Interchain Cysteine-Linked ADC Characterization by Mass Spectrometry.
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通过质谱法表征链间半胱氨酸连接的 ADC 的方法。
DOI：
10.1021/mp500614p
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发表时间：
2015
影响因子：
4.9
通讯作者：
L. Pan
中科院分区：
文献类型：
--
作者：
John F. Valliere;S. Hengel;L. Pan
治疗性抗体-药物缀合物（ADC）利用细胞毒性剂的细胞杀伤潜力和单克隆抗体的肿瘤靶向特异性来选择性地杀伤肿瘤细胞。近年来，已经见证了几种有前途的模式的发展，这些模式遵循基于ADC的疗法的相同基本原理，但采用独特的细胞毒性剂和缀合策略以实现治疗益处。ADC的复杂性和异质性对工业上依赖于生物制剂表征的一些常规分析方法提出了挑战。目前的审查将突出一些最近的方法学方法在质谱，弥合了差距时，传统的分析技术提供了一个不完整的图片ADC产品的质量。具体而言，我们将讨论保留和/或捕获有关ADC天然结构的信息的质谱方法，并提供对这些治疗分子的高阶结构（HOS）的独特见解。
Therapeutic antibody-drug conjugates (ADCs) harness the cell-killing potential of cytotoxic agents and the tumor targeting specificity of monoclonal antibodies to selectively kill tumor cells. Recent years have witnessed the development of several promising modalities that follow the same basic principles of ADC based therapies but which employ unique cytotoxic agents and conjugation strategies in order to realize therapeutic benefit. The complexity and heterogeneity of ADCs present a challenge to some of the conventional analytical methods that industry has relied upon for biologics characterization. This current review will highlight some of the more recent methodological approaches in mass spectrometry that have bridged the gap that is created when conventional analytical techniques provide an incomplete picture of ADC product quality. Specifically, we will discuss mass spectrometric approaches that preserve and/or capture information about the native structure of ADCs and provide unique insights into the higher order structure (HOS) of these therapeutic molecules.
影响因子：
6.8
作者：
Englander, SW;Sosnick, TR;Mayne, L
通讯作者：
Mayne, L
影响因子：
7.4
作者：
Zhang, Hao;Cui, Weidong;Wen, Jianzhong;Blankenship, Robert E.;Gross, Michael L.
通讯作者：
Gross, Michael L.