Diurnal control of intracellular distributions of PAS-Histidine kinase 1 and its interactions with partner proteins in the moss Physcomitrium patens.
Diurnal control of intracellular distributions of PAS-Histidine kinase 1 and its interactions with partner proteins in the moss Physcomitrium patens.
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PAS-组氨酸激酶 1 的细胞内分布的昼夜控制及其与苔藓小立碗藓中伙伴蛋白的相互作用。
DOI：
10.1016/j.bbrc.2022.05.070
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发表时间：
2022
影响因子：
3.1
通讯作者：
Aoki Setsuyuki
中科院分区：
文献类型：
--
作者：
Kikuchi Haruki;Yamashino Takafumi;Anami Shu;Suzuki Ryo;Sugita Mamoru;Aoki Setsuyuki
在多步磷酸传递（MSP）信号传导中，在接收到各种环境信号后，组氨酸激酶（HK）诱导自磷酸化和随后的磷酸转移到伴侣含组氨酸的磷酸转移蛋白（HPts）。最近，我们报道了立碗藓属（Physcomitrella）的两个含有Per-Arnt-Sim（PAS）结构域的HK（PHK 1和PHK 2）抑制红光诱导的原丝体组织分支，以及它们在黑暗中与细胞核中的伴侣HPt（HPt 1和HPt 2）相互作用，而在红光下也发生细胞质相互作用。在这里，我们证明PHK 1是昼夜调节的，即，它在夜间定位并与细胞核中的HPt 1和HPt 2相互作用，而这些活性在白天可逆地扩大并变为核质。在黑暗中，PHK 1仅在细胞核中与HPts相互作用，即使在主观的白天，也表明不涉及生物钟的内源性调节。这些结果表明，PHK 1是苔藓在日常时间尺度上适应光环境的调节因子。我们讨论了一个可能的调节机制的分支原丝体。
In multi-step phosphorelay (MSP) signaling, upon reception of various environmental signals, histidine kinases (HKs) induce autophosphorylation and subsequent phosphotransfer to partner histidine-containing phosphotransfer proteins (HPts). Recently, we reported that (i) two Per-Arnt-Sim (PAS) domain-containing HKs (PHK1 and PHK2) of the mossPhyscomitrium(Physcomitrella)patenssuppressed red light-induced branching of protonema tissue, and (ii) they interacted with partner HPts (HPt1 and HPt2) in the nucleus in the dark while cytoplasmic interactions also occurred under red light. Here we demonstrate that PHK1 is diurnally regulated,i.e., it is localized and interacts with HPt1 and HPt2 in the nucleus at night whereas these activities reversibly expand and become nucleocytoplasmic in the day. In the dark, PHK1 interacts with HPts only in the nucleus, even in subjective daytime, indicating that endogenous regulation by the circadian clock is not involved. These results suggest that PHK1 is a regulator of moss’ adaptation to a light environment on a daily timescale. We discuss a possible regulatory mechanism for the branching of protonema.
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影响因子：
1.6
作者：
Ishida, Kai;Yamashino, Takafumi;Mizuno, Takeshi
通讯作者：
Mizuno, Takeshi
细胞质光敏色素作用。
影响因子：
4.9
作者：
J. Rösler;Katharina Jaedicke;M. Zeidler
通讯作者：
M. Zeidler
影响因子：
56.9
作者：
Rensing, Stefan A.;Lang, Daniel;Boore, Jeffrey L.
通讯作者：
Boore, Jeffrey L.
含Chase结构域的受体在苔藓Physcomitrella patens的细胞分裂素反应中起着至关重要的作用。
影响因子：
6.9
作者：
von Schwartzenberg K;Lindner AC;Gruhn N;Šimura J;Novák O;Strnad M;Gonneau M;Nogué F;Heyl A
通讯作者：
Heyl A
影响因子：
4.1
作者：
Nishiyama, T;Hiwatashi, Y;Hasebe, M
通讯作者：
Hasebe, M