Functional analysis of Lycopene β-cyclase genes from two watermelon cultivars LSW-177 (red-flesh) and COS (pale-yellow flesh)
Functional analysis of Lycopene β-cyclase genes from two watermelon cultivars LSW-177 (red-flesh) and COS (pale-yellow flesh)
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两个西瓜品种LSW-177（红果肉）和COS（淡黄果肉）番茄红素β-环化酶基因的功能分析
DOI：
10.30848/pjb2020-1(19
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发表时间：
2020-01
影响因子：
1.2
通讯作者：
Feishi Luan
中科院分区：
文献类型：
--
作者：
Chuan Wu;Peng Gao;Shi Liu;Huiling Lv;Feishi Luan
类胡萝卜素广泛存在于植物组织中，具有多种功能。类胡萝卜素是植物光合作用中心特有的类胡萝卜素，其中大部分仅存在于彩色水果和蔬菜中。以前的研究结果表明，番茄红素环化酶是决定类胡萝卜素在果实，叶，花和根中的比例的基础。数量性状基因座（QTL）分析表明，一种酶与西瓜（Citrullus lanatus）的红色和非红色果肉有关，并且与番茄红素β-环化酶（lcyb）的功能有关。本研究对两个西瓜品种（LSW-177和COS）的lcyb酶基因进行了多态性分析。结果通过异源互补证实了大肠杆菌中两种lcyb蛋白的环化酶功能。这两种lcyb蛋白在226和435位氨基酸上存在差异，并具有将番茄红素环化为双环β-胡萝卜素的有效能力，表明这些氨基酸变异不影响大肠杆菌中lcyb酶的功能。杆菌此外，通过定点突变使酶失活的Ala 168转化为Glu 168，表明该残基是西瓜中lcyb功能的核心区域的一部分。这些结果将有助于进一步阐明番茄红素β-环化酶的作用机制。
Carotenoid pigments are extensively found in plant tissues with multifunction. The specific carotenoids have been classified in plant photosynthetic centers and most of them are exclusively abundant in colorful fruits and vegetables. Previous results show that lycopene cyclases are fundamental for determining the proportion of carotenoids in fruit, leaves, flowers and roots. A quantitative trait locus (QTL) analysis exhibited that an enzyme is responsible for red versus non-red flesh in watermelon (Citrullus lanatus) and also correlated with the function of lycopene β-cyclase (lcyb) with flesh colour. The current study was conducted to characterize the two polymorphic genes encoding lcyb enzymes, from two watermelon cultivars (LSW-177and COS) containin the red and pale-yellow flesh, respectively. Results had confirmed the cyclase function of the two lcyb proteins in an Escherichia coli by heterologous complementation. These twolcyb proteins were differential in the amino acids at the 226 and 435 positions and had the effective ability to cyclase lycopene into bicyclic β-carotene, showing that these amino acid variations did not influence the functionality of lcyb enzymes in E. coli. In addition, the conversion of Ala168 to Glu168 by site-directed mutagenesis which inactivated the enzyme, suggested that this residue was part of the core region for lcyb function in watermelon. The results presented here will help more to clarify the mechanism by which lycopene β-cyclase performs its function.
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DOI：
10.1007/s00122-015-2475-3
发表时间：
2015-05
期刊：
TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik
影响因子：
--
作者：
Suwarno WB;Pixley KV;Palacios-Rojas N;Kaeppler SM;Babu R
通讯作者：
Babu R
西瓜果实发育过程中转录组动态的表征：测序、组装、注释和基因表达谱
影响因子：
4.4
作者：
Guo S;Liu J;Zheng Y;Huang M;Zhang H;Gong G;He H;Ren Y;Zhong S;Fei Z;Xu Y
通讯作者：
Xu Y
DOI：
10.1055/s-2004-830990
发表时间：
2004
期刊：
Synthesis
影响因子：
--
作者：
A. Young
通讯作者：
A. Young
DOI：
--
发表时间：
2000-09
期刊：
CMAJ : Canadian Medical Association journal = journal de l'Association medicale canadienne
影响因子：
--
作者：
S. Agarwal;Akkinappally Venketeshwer Rao
通讯作者：
S. Agarwal;Akkinappally Venketeshwer Rao
影响因子：
64.8
作者：
Jaillon, Olivier;Aury, Jean-Marc;Wincker, Patrick
通讯作者：
Wincker, Patrick