Losartan treatment attenuates hindlimb unloading-induced atrophy in the soleus muscle of female rats via canonical TGF-β signaling.
Losartan treatment attenuates hindlimb unloading-induced atrophy in the soleus muscle of female rats via canonical TGF-β signaling.
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DOI：
10.1186/s12576-022-00830-8
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发表时间：
2022-03-09
影响因子：
2.3
通讯作者：
Naito, Hisashi
中科院分区：
文献类型：
--
作者：
Yoshihara, Toshinori;Takaragawa, Mizuki;Dobashi, Shohei;Naito, Hisashi
关键词：
我们研究了血管紧张素II 1型受体阻滞剂氯沙坦对比目鱼肌萎缩的保护作用。将年龄匹配的雄性和雌性Wistar大鼠进行后肢卸载，分别于第1天和第7天取出比目鱼肌进行分析。在后肢卸载后第7天，女性比目鱼肌的相对重量和肌纤维横截面积比男性减少得更多。氯沙坦部分保护女性免受肌肉萎缩的影响。通过Smad2/3磷酸化评估的典型转化生长因子-β信号通路的激活，在接受氯沙坦治疗的女性患者中较低，并与1天(肌原纤维)和7天(胞浆)的蛋白质泛素化水平降低有关。然而，在非典型性转化生长因子-β信号通路(p44/p42和p38MAPK磷酸化)中，男性和女性在卸载过程中没有观察到任何影响。我们的结果表明，氯沙坦对后肢卸载诱导的雌性大鼠比目鱼肌萎缩有部分保护作用，可能与减少典型的转化生长因子-β信号有关。网上版载有补充材料，可在10.1186/s12576-022-00830-8查阅。
We investigated the protective effect of losartan, an angiotensin II type 1 receptor blocker, on soleus muscle atrophy. Age-matched male and female Wistar rats were subjected to hindlimb unloading, and the soleus muscle was removed on days 1 and 7 for analysis. Females showed greater reductions in relative weight and myofiber cross-sectional area of the soleus muscle than males on day 7 post-hindlimb unloading. Losartan partially protected females against muscle atrophy. Activation of the canonical TGF-β signaling pathway, assessed via Smad2/3 phosphorylation, was lower in females following losartan treatment and associated with lower levels of protein ubiquitination after 1 (myofibril) and 7 (cytosol) days of unloading. However, no effect was observed in non-canonical TGF-β signaling (p44/p42 and p38 MAPK phosphorylation) in males or females during unloading. Our results suggest that losartan provides partial protection against hindlimb unloading-induced soleus muscle atrophy in female rats, possibly associated with decreased canonical TGF-β signaling. The online version contains supplementary material available at 10.1186/s12576-022-00830-8.
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影响因子：
15.9
作者：
TIAO, G;FAGAN, JM;HASSELGREN, PO
通讯作者：
HASSELGREN, PO
DOI：
10.1152/ajpregu.00761.2007
发表时间：
2008-03-01
影响因子：
2.8
作者：
Trappe, Scott;Creer, Andrew;Trappe, Todd
通讯作者：
Trappe, Todd
影响因子：
3.3
作者：
Trappe, Scott;Creer, Andrew;Trappe, Todd
通讯作者：
Trappe, Todd
影响因子：
2.3
作者：
Yoshihara, Toshinori;Natsume, Toshiharu;Naito, Hisashi
通讯作者：
Naito, Hisashi
影响因子：
82.9
作者：
Cohn, Ronald D.;van Erp, Christel;Dietz, Harry C.
通讯作者：
Dietz, Harry C.