CD14-positive extracellular vesicles in bronchoalveolar lavage fluid as a new biomarker of acute respiratory distress syndrome.
CD14-positive extracellular vesicles in bronchoalveolar lavage fluid as a new biomarker of acute respiratory distress syndrome.
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DOI：
10.1152/ajplung.00052.2022
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发表时间：
2022-04-01
期刊：
影响因子：
--
通讯作者：
Thickett DR
中科院分区：
文献类型：
--
作者：
Mahida RY;Price J;Lugg ST;Li H;Parekh D;Scott A;Harrison P;Matthay MA;Thickett DR
近年来的研究表明，细胞外囊泡（EVs）可能在急性呼吸窘迫综合征（ARDS）的发病机制中发挥作用。EV已被确定为其他肺部疾病中疾病严重程度和预后的潜在生物标志物。我们试图描述ARDS患者支气管肺泡灌洗液（BAL）中EV表型的特征，并确定BAL EV是否可用作ARDS的潜在生物标志物。从脓毒症伴和不伴ARDS的患者以及术后食管切除术患者（其中一个亚组后来在住院期间发生ARDS）中采集BAL。BAL EV在大小、数量和起源细胞方面进行表征。脓毒症相关ARDS患者的CD 14 +/CD 81+单核细胞来源的BALEV数量显著高于脓毒症无ARDS患者（P = 0.015）。然而，在食管切除术后发生ARDS的患者中观察到匡威的情况（P = 0.003）。与未发生ARDS的食管切除术患者相比，发生ARDS的食管切除术患者的CD 31 +/CD 63+和CD 31 +/CD 81+内皮源性BAL EV也升高（P ≤ 0. 02）。需要进一步的研究来确定CD 31 + BAL EV是否可能是食管切除术患者中ARDS的预测生物标志物。在重症监护病房入院后30天内死亡的脓毒症相关ARDS患者中，CD 14 +/CD 81 + BAL EV数量显著较高（P = 0.027）。因此，CD 14 +/CD 81 + BAL EV是脓毒症相关ARDS中疾病严重程度和死亡率的潜在生物标志物。这些发现为进一步阐明这些EV在ARDS发病机制中的作用提供了动力。
Recent studies have indicated that extracellular vesicles (EVs) may play a role in the pathogenesis of acute respiratory distress syndrome (ARDS). EVs have been identified as potential biomarkers of disease severity and prognosis in other pulmonary diseases. We sought to characterize the EV phenotype within bronchoalveolar lavage (BAL) fluid of patients with ARDS, and to determine whether BAL EV could be used as a potential biomarker in ARDS. BAL was collected from patients with sepsis with and without ARDS, and from esophagectomy patients postoperatively (of whom a subset later developed ARDS during hospital admission). BAL EVs were characterized with regard to size, number, and cell of origin. Patients with sepsis-related ARDS had significantly higher numbers of CD14+/CD81+ monocyte-derived BAL EV than patients with sepsis without ARDS (P = 0.015). However, the converse was observed in esophagectomy patients who later developed ARDS (P = 0.003). Esophagectomy patients who developed ARDS also had elevated CD31+/CD63+ and CD31+/CD81+ endothelial-derived BAL EV (P ≤ 0.02) compared with esophagectomy patients who did not develop ARDS. Further studies are required to determine whether CD31+ BAL EV may be a predictive biomarker for ARDS in esophagectomy patients. CD14+/CD81+ BAL EV numbers were significantly higher in those patients with sepsis-related ARDS who died during the 30 days following intensive care unit admission (P = 0.027). Thus, CD14+/CD81+ BAL EVs are a potential biomarker for disease severity and mortality in sepsis-related ARDS. These findings provide the impetus to further elucidate the contribution of these EVs to ARDS pathogenesis.
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影响因子：
8.8
作者：
Parekh D;Dancer RCA;Scott A;D'Souza VK;Howells PA;Mahida RY;Tang JCY;Cooper MS;Fraser WD;Tan L;Gao F;Martineau AR;Tucker O;Perkins GD;Thickett DR
通讯作者：
Thickett DR
影响因子：
5.4
作者：
Shen, Guifen;Krienke, Stefan;Tykocinski, Lars-Oliver
通讯作者：
Tykocinski, Lars-Oliver
影响因子：
76.2
作者：
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通讯作者：
Matthay, Michael A.
DOI：
10.1165/rcmb.2017-0393oc
发表时间：
2018-07-01
影响因子：
6.4
作者：
Mitra, Srabani;Exline, Matthew;Sarkar, Anasuya
通讯作者：
Sarkar, Anasuya
DOI：
10.1152/ajplung.00362.2020
发表时间：
2021-02-01
影响因子：
4.9
作者：
Bazzan, Erica;Radu, Claudia Maria;Saetta, Marina
通讯作者：
Saetta, Marina