Cell type-specific activation of AKT and ERK signaling pathways by small negatively-charged magnetic nanoparticles.
Cell type-specific activation of AKT and ERK signaling pathways by small negatively-charged magnetic nanoparticles.
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通过小负荷磁性纳米颗粒对Akt和ERK信号通路的细胞类型特异性激活。
DOI：
10.1038/srep00868
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发表时间：
2012
影响因子：
4.6
通讯作者：
Mahmoudi, Morteza
中科院分区：
文献类型：
--
作者：
Rauch, Jens;Kolch, Walter;Mahmoudi, Morteza
纳米粒子（NPs）与生物体的相互作用已成为公众和科学争论的焦点，因为它们在生物医学中的潜在广泛应用，但也因为不必要的副作用。在这里，我们表明，具有不同表面涂层的超顺磁性氧化铁纳米粒子（SPION）可以不同地影响信号转导途径。使用乳腺和结肠来源的细胞系的同基因对，我们发现SPION对ERK和AKT信号通路的刺激选择性地依赖于细胞类型和SPION类型。一般来说，Ras突变的细胞比非突变的细胞反应更好。小的带负电荷的SPION（snSPION）以与表皮生长因子（EGF）相似的程度激活ERK，并且使用相同的上游信号传导组分，包括EGF受体的激活。重要的是，snSPION刺激Ras转化的乳腺上皮细胞的增殖效率与EGF一样，表明NP可以模拟生理生长因子。
The interaction of nanoparticles (NPs) with living organisms has become a focus of public and scientific debate due to their potential wide applications in biomedicine, but also because of unwanted side effects. Here, we show that superparamagnetic iron oxide NPs (SPIONs) with different surface coatings can differentially affect signal transduction pathways. Using isogenic pairs of breast and colon derived cell lines we found that the stimulation of ERK and AKT signaling pathways by SPIONs is selectively dependent on the cell type and SPION type. In general, cells with Ras mutations respond better than their non-mutant counterparts. Small negatively charged SPIONs (snSPIONs) activated ERK to a similar extent as epidermal growth factor (EGF), and used the same upstream signaling components including activation of the EGF receptor. Importantly, snSPIONs stimulated the proliferation of Ras transformed breast epithelial cells as efficiently as EGF suggesting that NPs can mimic physiological growth factors.
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