Lipid requirements for coupled cytochrome oxidase vesicles.
Lipid requirements for coupled cytochrome oxidase vesicles.
复制标题
偶联细胞色素氧化酶囊泡的脂质需求。
DOI：
10.1021/bi00277a036
复制
发表时间：
1983
期刊：
影响因子：
2.9
通讯作者：
P. Cullis
中科院分区：
文献类型：
--
作者：
T. D. Madden;M. Hope;P. Cullis
Cytochrome c oxidase has been reconstituted with two synthetic phospholipids, dioleoylphosphatidylcholine and dioleoylphosphatidylethanolamine. Vesicles prepared from either of these two lipids alone showed no stimulation of enzyme activity upon addition of carbonyl cyanide (trifluoromethoxy)phenylhydrazone and valinomycin, indicating that they were leaky to small ions. However, when mixtures of the two lipids were used for the reconstitution, tightly coupled vesicles could be obtained. The coupling ratio was dependent upon the ratio of dioleoylphosphatidylcholine to dioleoylphosphatidylethanolamine and also on the lipid-to-protein ratio. Maximal rates of enzyme activity were not significantly different with different lipid mixtures. The results are discussed in terms of both the size distribution of the reconstituted vesicles and the possible requirement for a variety of lipid species to ensure tight sealing at the lipid-protein interface.
影响因子：
2.9
作者：
MIMMS, LT;ZAMPIGHI, G;REYNOLDS, JA
通讯作者：
REYNOLDS, JA
重建肌质网膜的功能特征作为脂质与蛋白质比率的函数。
影响因子：
3.4
作者：
Herbette,L;Scarpa,A;Blasie,JK;Bauer,DR;Wang,CT;Fleischer,S
通讯作者：
Fleischer,S