Relationship of the Esophageal Microbiome and Tissue Gene Expression and Links to the Oral Microbiome: A Randomized Clinical Trial.
Relationship of the Esophageal Microbiome and Tissue Gene Expression and Links to the Oral Microbiome: A Randomized Clinical Trial.
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DOI：
10.14309/ctg.0000000000000235
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发表时间：
2020-12
影响因子：
3.6
通讯作者：
Abrams JA
中科院分区：
文献类型：
--
作者：
Annavajhala MK;May M;Compres G;Freedberg DE;Graham R;Stump S;Que J;Korem T;Uhlemann AC;Abrams JA
虽然微生物组在各种食道疾病中都会改变，但没有直接证据表明口腔或食道微生物组与潜在的食道组织之间存在联系。在这里，我们的目标是通过使用抗菌漱口水来改变食道微生物组和组织基因表达来解决这些差距。在这项随机对照试验中，计划接受内窥镜检查的临床指征患者在内窥镜检查前2周使用洗必泰含漱液或不治疗。在基线和随访时收集口腔拭子和唾液，并在内窥镜检查当天采集食道样本。用16S rRNA基因测序分析微生物组，用RNA-Seq法确定食道组织基因的表达。20名受试者入选并纳入分析。在个体内，口腔和食道微生物组组成显著相关。洗必泰治疗与几个食道细菌分类群的相对丰度和食道中基因表达的显著变化有关，包括Periostin、Claudin-18、趋化因子CXCL1和CXCL13以及肿瘤坏死因子受体超家族的几个成员的减少。食道中的嗜血杆菌属的一个分类群也与组织基因表达的显著变化有关。口腔和食道微生物群在个体内密切相关，食道微生物群的变化与组织基因表达的变化密切相关。食道微生物群可能是影响嗜酸性食管炎、胃食道反流和Barrett‘s食道等疾病的发病机制和预后的重要辅助因素。
Although the microbiome is altered in various esophageal diseases, there is no direct evidence for a link between the oral or esophageal microbiome and underlying esophageal tissue. Here, we aimed to address these gaps through use of an antimicrobial mouth rinse to modify the esophageal microbiome and tissue gene expression. In this randomized controlled trial, patients scheduled to undergo endoscopy for clinical indications used chlorhexidine mouth rinse or no treatment for 2 weeks before endoscopy. Oral swabs and saliva were collected at baseline and at follow-up, and the esophagus was sampled on the day of endoscopy. The microbiome was analyzed by 16S rRNA gene sequencing, and esophageal tissue gene expression was ascertained by RNA-Seq. Twenty subjects were enrolled and included in the analyses. Within individuals, the oral and esophageal microbiome composition was significantly correlated. Chlorhexidine treatment associated with significant alterations to the relative abundance of several esophageal bacterial taxa, and to expression of genes in the esophagus including reductions in periostin, claudin-18, chemokines CXCL1 and CXCL13, and several members of the tumor necrosis factor receptor superfamily. A taxon in genus Haemophilus in the esophagus also associated with significant changes in tissue gene expression. The oral and esophageal microbiomes are closely related within individuals, and esophageal microbiome alterations correlate with tissue gene expression changes. The esophageal microbiome may act as an important cofactor that influences pathogenesis and outcomes of diseases such as eosinophilic esophagitis, gastroesophageal reflux, and Barrett's esophagus.
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影响因子：
3.8
作者：
Snider, Erik J.;Compres, Griselda;Abrams, Julian A.
通讯作者：
Abrams, Julian A.
DOI：
10.1152/ajpgi.00158.2007
发表时间：
2007-12-01
影响因子：
4.5
作者：
Jovov, Biljana;Van Itallie, Christina M.;Orlando, Roy C.
通讯作者：
Orlando, Roy C.
影响因子：
15.9
作者：
Tomkovich, Sarah;Dejea, Christine M.;Sears, Cynthia L.
通讯作者：
Sears, Cynthia L.
影响因子：
15.5
作者：
Benitez AJ;Hoffmann C;Muir AB;Dods KK;Spergel JM;Bushman FD;Wang ML
通讯作者：
Wang ML
影响因子：
3.6
作者：
Arias Á;Vicario M;Bernardo D;Olalla JM;Fortea M;Montalban-Arques A;Martínez-Fernández P;González-Castro AM;Mota-Huertas T;Arias-González L;Lucendo AJ
通讯作者：
Lucendo AJ