Inflammation-associated microbiota in pediatric eosinophilic esophagitis.
Inflammation-associated microbiota in pediatric eosinophilic esophagitis.
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DOI：
10.1186/s40168-015-0085-6
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发表时间：
2015
期刊：
影响因子：
15.5
通讯作者：
Wang ML
中科院分区：
文献类型：
--
作者：
Benitez AJ;Hoffmann C;Muir AB;Dods KK;Spergel JM;Bushman FD;Wang ML
嗜酸性粒细胞性食管炎（EoE）是一种以嗜酸性粒细胞为主的食管炎症为特征的过敏性疾病，可以通过限制食物抗原来改善。尽管最近的研究表明，饮食组成的改变可能会改变远端肠道微生物群，但目前对EoE背景下限制饮食对口腔和食管微环境微生物群落的影响知之甚少。我们假设EoE患者的口腔和食管微生物组与非EoE对照组不同，这些差异对应于食管炎症的变化，并且有针对性的治疗性饮食干预可能会影响群落结构。使用16S rRNA基因测序，我们利用35名非EoE儿童对照的口腔拭子和食管活检来表征口腔和食管微环境的细菌组成，并将该队列与33名EoE儿童受试者在确定的饮食改变前后进行纵向研究的样本进行比较。与口腔样品相比，食管样品中厚壁菌门更丰富。所有EoE食管微生物群与非EoE对照组相比，细菌群落的比例存在显著差异，在EoE队列中，变形菌门（包括奈瑟菌和棒状杆菌）富集，而在非EoE对照组中，厚壁菌门占优势。我们发现活跃炎症的EoE活检与非EoE对照之间存在统计学上的显著差异。总体而言，尽管有针对性的饮食干预并没有导致口腔或食管微生物群的显著差异，但重新引入高过敏性食物会导致食管中Ganulicatella和弯曲杆菌属的富集。综上所述，EoE患者的食管微生物组与非EoE对照组不同，在活动性过敏性炎症期间差异最大。本文的在线版本（doi:10.1186/s40168-015-0085-6）包含补充材料，可供授权用户使用。
Eosinophilic esophagitis (EoE) is an allergic disorder characterized by eosinophil-predominant esophageal inflammation, which can be ameliorated by food antigen restriction. Though recent studies suggest that changes in dietary composition may alter the distal gut microbiome, little is currently known about the impact of a restricted diet upon microbial communities of the oral and esophageal microenvironments in the context of EoE. We hypothesize that the oral and esophageal microbiomes of EoE patients are distinct from non-EoE controls, that these differences correspond to changes in esophageal inflammation, and that targeted therapeutic dietary intervention may influence community structure. Using 16S rRNA gene sequencing, we characterized the bacterial composition of the oral and esophageal microenvironments using oral swabs and esophageal biopsies from 35 non-EoE pediatric controls and compared this cohort to samples from 33 pediatric EoE subjects studied in a longitudinal fashion before and after defined dietary changes. Firmicutes were more abundant in esophageal samples compared to oral. Proportions of bacterial communities were significantly different comparing all EoE esophageal microbiota to non-EoE controls, with enrichment of Proteobacteria, including Neisseria and Corynebacterium in the EoE cohort, and predominance of the Firmicutes in non-EoE control subjects. We detected a statistically significant difference between actively inflamed EoE biopsies and non-EoE controls. Overall, though targeted dietary intervention did not lead to significant differences in either oral or esophageal microbiota, reintroduction of highly allergenic foods led to enrichment in Ganulicatella and Campylobacter genera in the esophagus. In conclusion, the esophageal microbiome in EoE is distinct from that of non-EoE controls, with maximal differences observed during active allergic inflammation. The online version of this article (doi:10.1186/s40168-015-0085-6) contains supplementary material, which is available to authorized users.
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影响因子：
82.9
作者：
通讯作者：
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影响因子：
64.8
作者：
Alenghat, Theresa;Osborne, Lisa C.;Saenz, Steven A.;Kobuley, Dmytro;Ziegler, Carly G. K.;Mullican, Shannon E.;Choi, Inchan;Grunberg, Stephanie;Sinha, Rohini;Wynosky-Dolfi, Meghan;Snyder, Annelise;Giacomin, Paul R.;Joyce, Karen L.;Hoang, Tram B.;Bewtra, Meenakshi;Brodsky, Igor E.;Sonnenberg, Gregory F.;Bushman, Frederic D.;Won, Kyoung-Jae;Lazar, Mitchell A.;Artis, David
通讯作者：
Artis, David
DOI：
10.1038/ismej.2012.8
发表时间：
2012-08
期刊：
The ISME journal
影响因子：
--
作者：
通讯作者：
--
影响因子：
3.7
作者：
Holodniy, Mark;Brown, Sheldon T.;Darbyshire, Janet
通讯作者：
Darbyshire, Janet
影响因子：
14.2
作者：
Noval Rivas, Magali;Burton, Oliver T.;Wise, Petra;Zhang, Yu-qian;Hobson, Suejy A.;Lloret, Maria Garcia;Chehoud, Christel;Kuczynski, Justin;DeSantis, Todd;Warrington, Janet;Hyde, Embriette R.;Petrosino, Joseph F.;Gerber, Georg K.;Bry, Lynn;Oettgen, Hans C.;Mazmanian, Sarkis K.;Chatila, Talal A.
通讯作者：
Chatila, Talal A.