Polarized SCAR and the Arp2/3 complex regulate apical cortical remodeling in asymmetrically dividing neuroblasts.
Polarized SCAR and the Arp2/3 complex regulate apical cortical remodeling in asymmetrically dividing neuroblasts.
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DOI：
10.1016/j.isci.2023.107129
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发表时间：
2023-07-21
期刊：
影响因子：
5.8
通讯作者：
Baum, Buzz
中科院分区：
文献类型：
--
作者：
Cazzagon, Giulia;Roubinet, Chantal;Baum, Buzz
尽管在对称性和非对称性细胞分裂中，Forin核的肌动球蛋白皮质已经被证明驱动伴随着动物细胞分裂的细胞形状的变化，但皮质Arp2/3核的肌动蛋白网络的有丝分裂作用尚不清楚。在这里，我们使用不对称分裂的果蝇神经干细胞作为模型系统，当神经母细胞进入有丝分裂时，我们识别出在神经母细胞顶端皮质形成的膜突起池。值得注意的是，这些位于顶端的局部突起富含SCAR，并依赖于SCAR和Arp2/3复合体的形成。由于受损的SCAR或Arp2/3复合体推迟了后期肌球蛋白II的顶端清除，并导致细胞质分裂时皮质不稳定，这些数据表明顶端分枝的肌动蛋白细丝网络在微调肌球蛋白皮质方面发挥了作用，从而能够精确控制不对称细胞分裂期间细胞形状的变化。极化的SCAR和Arp2/3活性为不对称飞行干细胞分裂奠定了基础Arp2/3控制细胞进入后期的皮质重塑动力学取决于极性蛋白Pins Arp2/3和SCAR限制细胞进入后期时肌球蛋白的顶端积累
Although the formin-nucleated actomyosin cortex has been shown to drive the changes in cell shape that accompany animal cell division in both symmetric and asymmetric cell divisions, the mitotic role of cortical Arp2/3-nucleated actin networks remain unclear. Here using asymmetrically dividing Drosophila neural stem cells as a model system, we identify a pool of membrane protrusions that form at the apical cortex of neuroblasts as they enter mitosis. Strikingly, these apically localized protrusions are enriched in SCAR, and depend on SCAR and Arp2/3 complexes for their formation. Because compromising SCAR or the Arp2/3 complex delays the apical clearance of Myosin II at the onset of anaphase and induces cortical instability at cytokinesis, these data point to a role for an apical branched actin filament network in fine-tuning the actomyosin cortex to enable the precise control of cell shape changes during an asymmetric cell division. Polarized SCAR and Arp2/3 activity set the stage for asymmetric fly stem cell divisions Arp2/3 controls the dynamics of cortical remodeling in cells entering anaphase Apical SCAR recruitment and protrusion formation depend on the polarity protein Pins Arp2/3 and SCAR limit the apical accumulation of Myosin as cells enter anaphase Biological sciences; Molecular biology; Neuroscience; Cell biology
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DOI：
10.1038/nrm2867
发表时间：
2010-04
期刊：
Nature reviews. Molecular cell biology
影响因子：
--
作者：
通讯作者：
--
Wnt 信号传导极化皮质肌动蛋白聚合以增加子细胞不对称性
影响因子：
33.5
作者：
Chai Y;Tian D;Zhu Z;Jiang Y;Huang S;Wu D;Ou G;Li W
通讯作者：
Li W
影响因子：
21.3
作者：
Morton, WM;Ayscough, KR;McLaughlin, PJ
通讯作者：
McLaughlin, PJ
影响因子：
7.7
作者：
Oon, Chet Huan;Prehoda, Kenneth E.
通讯作者：
Prehoda, Kenneth E.
影响因子：
3.3
作者：
Connell M;Cabernard C;Ricketson D;Doe CQ;Prehoda KE
通讯作者：
Prehoda KE