Comparison of the cytokine and chemokine dynamics of the early inflammatory response in models of burn injury and infection.
Comparison of the cytokine and chemokine dynamics of the early inflammatory response in models of burn injury and infection.
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DOI：
10.1016/j.cyto.2011.05.010
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发表时间：
2011-09
期刊：
影响因子：
3.8
通讯作者：
Androulakis, Ioannis P.
中科院分区：
文献类型：
--
作者：
Orman, Mehmet A.;Nguyen, Tung T.;Ierapetritou, Marianthi G.;Berthiaume, Francois;Androulakis, Ioannis P.
炎症反应及其随后的消退是源于损伤或感染部位的一系列非常复杂的事件的结果。当反应严重且持续时，可能会出现系统性炎症反应综合征，这与严重衰弱的全身高分解代谢状态有关。这种由细胞因子和趋化因子介导的复杂行为需要进一步探索，以更好地了解其系统特性，并潜在地确定可以同时解决的多个目标。在这种情况下，血清细胞因子和趋化因子的短期反应分析了两种类型的损伤：接受20%体表面积（TBSA）的“无菌”皮肤背部烧伤的大鼠；大鼠接受盲肠结扎和穿刺治疗（CLP）诱导感染。考虑到与对照组对应的基线的时间变异性，我们探索了曲线下面积（AUC）的概念来评估细胞因子和趋化因子的动态反应。烧伤组和CLP组均有MCP-1、GROK/KC、IL-12、IL-18、IL-10的表达。IL-10浓度仅在烧伤组升高，Eotaxin仅在CLP组升高。CLP组和假CLP组瘦素和IP-1浓度均降低。通过搜索细胞因子/趋化因子基因的启动子区域，探索循环蛋白介质与调节细胞因子/趋化因子基因表达的推定转录因子之间的联系，从而根据动态数据表征和区分炎症反应。结合多种来源和生物信息学工具，确定了对损伤类型和程度敏感的介质，并提供了假定的调节机制。这对于在分子水平上更好地理解可用于调节炎症状态的重要调控点是必不可少的。
The inflammatory response, and its subsequent resolution, are the result of a very complex cascade of events originating at the site of injury or infection. When the response is severe and persistent, Systemic Inflammatory Response Syndrome can set in, which is associated with a severely debilitating systemic hypercatabolic state. This complex behavior, mediated by cytokines and chemokines, needs to be further explored to better understand its systems properties and potentially identify multiple targets that could be addressed simultaneously. In this context, short term responses of serum cytokines and chemokines were analyzed in two types of insults: rats receiving a “sterile” cutaneous dorsal burn on 20% of the total body surface area (TBSA); rats receiving a cecum ligation and puncture treatment (CLP) to induce infection. Considering the temporal variability observed in the baseline corresponding to the control group, the concept of area under the curve (AUC) was explored to assess the dynamic responses of cytokines and chemokines. MCP-1, GROK/KC, IL-12, IL-18 and IL-10 were observed in both burn and CLP groups. While IL-10 concentration was only increased in the burn group, Eotaxin was only elevated in CLP group. It was also observed that Leptin and IP-1 concentrations were decreased in both CLP and sham-CLP groups. The link between the circulating protein mediators and putative transcription factors regulating the cytokine/chemokine gene expression was explored by searching the promoter regions of cytokine/chemokine genes in order to characterize and differentiate the inflammatory responses based on the dynamic data. Integrating multiple sources together with the bioinformatics tools identified mediators sensitive to type and extent of injury, and provided putative regulatory mechanisms. This is essential to gain a better understanding for the important regulatory points that can be used to modulate the inflammatory state at molecular level.
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影响因子：
56.9
作者：
Carninci, P;Kasukawa, T;Hayashizaki, Y
通讯作者：
Hayashizaki, Y
影响因子：
3.8
作者：
Finnerty, Celeste C.;Przkora, Rene;Herndon, David N.;Jeschke, Marc G.
通讯作者：
Jeschke, Marc G.
影响因子：
11.8
作者：
Feezor, RJ;Cheng, A;Moldawer, LL
通讯作者：
Moldawer, LL
影响因子：
3.8
作者：
Banta, Scott;Vemula, Murali;Yarmush, Martin L.
通讯作者：
Yarmush, Martin L.
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y