Articular cartilage vesicles contain RNA.
Articular cartilage vesicles contain RNA.
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DOI：
10.1016/j.bbrc.2009.08.038
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发表时间：
2009-10-23
影响因子：
3.1
通讯作者：
Rosenthal, Ann K.
中科院分区：
文献类型：
--
作者：
Mitton, Elizabeth;Gohr, Claudia M.;McNally, Mark T.;Rosenthal, Ann K.
关键词：
关节软骨基质囊泡（ACV）是一种小的膜结合细胞外细胞器，参与了骨关节炎关节软骨的病理矿化。ACV也存在于正常软骨中，尽管它们除了矿化之外没有已知的功能。最近，在肥大细胞来源的细胞外囊泡中发现了RNA，这表明这种囊泡可能携带细胞间的编码信息。我们发现，来自正常猪和人关节软骨和原代软骨细胞条件培养基的ACV含有1 μg RNA/80μg ACV蛋白。没有检测到DNA。ACV RNA的RT-PCR证实了因子XIIIA、II型转氨酶、II型胶原、聚集蛋白聚糖、ANKH和GAPDH的全长mRNA的存在。完整的无环鸟苷中的RNA对核糖核酸酶具有抗性，尽管无环鸟苷制剂含有可测量水平的活性核糖核酸酶。值得注意的是，ACV中放射性标记的RNA可通过共孵育转移到未标记的软骨细胞中，并引起软骨细胞酶和蛋白质水平的变化。ACV含有mRNA的证明表明，它们可能起到在关节细胞之间穿梭遗传信息的作用，并表明这些结构在关节软骨中具有新的功能。
Small membrane-bound extracellular organelles known as articular cartilage matrix vesicles (ACVs) participate in pathologic mineralization in osteoarthritic articular cartilage. ACVs are also present in normal cartilage, although they have no known functions other than mineralization. Recently, RNA was identified in extracellular vesicles derived from mast cells, suggesting that such vesicles might carry coding information from cell to cell. We found that ACVs from normal porcine and human articular cartilage and primary chondrocyte conditioned media contained 1 μg RNA/80μg ACV protein. No DNA could be detected. RT-PCR of ACV RNA demonstrated the presence of full length mRNAs for factor XIIIA, type II transglutaminase, collagen II, aggrecan, ANKH and GAPDH. RNA in intact ACVs was resistant to RNase, despite the fact that ACV preparations contained measurable levels of active RNases. Significantly, radiolabelled RNA in ACVs could be transferred to unlabelled chondrocytes by co-incubation and produced changes in levels of chondrocyte enzymes and proteins. The demonstration that ACVs contain mRNAs suggests that they may function to shuttle genetic information between articular cells and indicate novel functions for these structures in articular cartilage.
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