Conformational perturbation due to an extra adenosine in a self‐complementary oligodeoxynucleotide duplex
Conformational perturbation due to an extra adenosine in a self‐complementary oligodeoxynucleotide duplex
复制标题
自互补寡脱氧核苷酸双链体中额外腺苷引起的构象扰动
作者：
Siddhartha Roy;V. Sklenar;E. Appella;J. S. Cohen
用核磁共振光谱研究了寡脱氧核苷酸十五聚体d（CGCGAAATTTACGCG）的构象，除了11位的额外腺苷核苷酸外，该构象是自互补的。发现该寡聚体在溶液中主要以双链体形式存在，而不是如先前对类似的13-mer序列所观察到的发夹环形式。核Overhauser效应表明B-型构象被额外的A碱基破坏，在双链体内形成楔形，产生弯曲的连接和整体S-样结构。使用二维1H-31 P相关方法分配了低场位移的磷酸盐共振，发现其为P12 - 13。这一结果和其他结果表明，额外的A导致构象扭曲在一定距离沿着双链体结构。
The conformation of an oligodeoxynucleotide pentadecamer, d(CGCGAAATTTACGCG), self‐complementary except for an additional adenosine nucleotide at position 11, has been investigated with nmr spectroscopy. This oligomer was found to exist predominantly in the duplex form in solution rather than in the hairpin loop form, as observed previously for an analogous 13‐mer sequence. Nuclear Overhauser effects indicate that the B‐form conformation is disrupted by the extra A base, which forms a wedge within the duplex, producing a bent junction and an overall S‐like structure. A downfield‐shifted phosphate resonance was assigned using a two‐dimensional 1H–31P correlation method, and was found to be P12‐13. This and other results indicate that the extra A causes conformational distortions at some distance along the duplex structure.
d(C-G-C-G-A-A-T-T-C-G-C-G) 双螺旋和包含 G 的两个相似螺旋中亚氨基质子交换的动力学。
影响因子：
2.9
作者：
Pardi,A;Morden,KM;Patel,DJ;TinocoJr,I
通讯作者：
TinocoJr,I