Reduced repair of potentially lethal radiation damage in glutathione synthetase-deficient human fibroblasts after X-irradiation.
Reduced repair of potentially lethal radiation damage in glutathione synthetase-deficient human fibroblasts after X-irradiation.
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X 射线照射后谷胱甘肽合成酶缺陷的人成纤维细胞中潜在致命辐射损伤的修复减少。
DOI：
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
L. Révész
中科院分区：
文献类型：
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作者：
J. Midander;P. Deschavanne;D. Debieu;E. Malaise;L. Révész
利用缺乏谷胱甘肽合成酶的人成纤维细胞菌株和相关的对照菌株，研究了谷胱甘肽（GSH）在修复潜在致命损伤（PLD）中的作用，通过立即或照射后24小时的细胞镀确定细胞的存活率。在缺氧或缺氧照射后，两种细胞株都能修复辐射引起的损伤。然而，在缺氧条件下，熟练细胞修复PLD的能力与缺氧条件下一样，而缺陷细胞在缺氧条件下修复PLD的能力低于缺氧条件下。因此，无论是立即镀还是24 h后镀，熟练细胞的氧增强比（o.e.r）是相似的（分别为2.0和2.13）。相比之下，缺陷细胞的o.e.r.在照射24小时后镀的细胞比在照射后立即镀的细胞要低（1.16比1.55）。结果表明，谷胱甘肽参与PLD修复，特别是在缺氧条件下辐射引起的损伤修复中。
Using a human fibroblast strain deficient in glutathione synthetase and a related proficient control strain, the role of glutathione (GSH) in repair of potentially lethal damage (PLD) has been investigated in determining survival by plating cells immediately or 24 h after irradiation. After oxic or hypoxic irradiation, both cell strains repair radiation-induced damage. However, under hypoxic conditions, the proficient cells repair PLD as well as under oxic conditions while the deficient cells repair less PLD after irradiation under hypoxic than under oxic conditions. Therefore, the oxygen enhancement ratio (o.e.r.) for proficient cells is similar whether the cells are plated immediately or 24 h later (2.0 and 2.13, respectively). In contrast, the o.e.r. for deficient cells is lower when the cells are plated 24 h after irradiation than when they are plated immediately thereafter (1.16 as compared to 1.55). The results indicate that GSH is involved in PLD repair and, in particular, in the repair of damage induced by radiation delivered under hypoxic conditions.
D,L-丁硫氨酸-S,R-亚磺酰亚胺对中国仓鼠 V79 细胞硫醇水平和氧效应的影响。
DOI：
10.1016/0360-3016(84)90325-0
发表时间：
1984
期刊：
International journal of radiation oncology, biology, physics
影响因子：
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作者：
Astor,MB;Hall,EJ;Biaglow,JE;Hartog,B
通讯作者：
Hartog,B
影响因子：
56.9
作者：
BUMP, EA;YU, NY;BROWN, JM
通讯作者：
BROWN, JM