A simple method for deriving functional MSCs and applied for osteogenesis in 3D scaffolds.
A simple method for deriving functional MSCs and applied for osteogenesis in 3D scaffolds.
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一种衍生功能性 MSC 并应用于 3D 支架中成骨的简单方法。
DOI：
10.1038/srep02243
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发表时间：
2013
影响因子：
4.6
通讯作者：
Bunger, Cody
中科院分区：
文献类型：
--
作者：
Zou, Lijin;Luo, Yonglun;Chen, Muwan;Wang, Gang;Ding, Ming;Petersen, Charlotte Christie;Kang, Ran;Dagnaes-Hansen, Frederik;Zeng, Yuanlin;Lv, Nonghua;Ma, Qing;Le, Dang Q. S.;Besenbacher, Flemming;Bolund, Lars;Jensen, Thomas G.;Kjems, Jorgen;Pu, William T.;Bunger, Cody
我们描述了一种简单的骨工程方法，使用从人诱导多能干细胞(HIPS-MSCs)来源的间充质干细胞(HIPS-MSCs)衍生的可生物降解支架。HIPS-MSCs表达间充质标志物(CD90、CD73和CD105)，具有成骨、成脂和成软骨三系分化的多能性，失去多能性--如标记Oct3/4和TRA-1-81的缺失--和致瘤性。然而，这些iPS-MSCs的标志物NANOG仍然是阳性的。我们进一步探讨了HIPS-MSCs在人工合成的聚己内酯(PCL)支架和天然聚合物透明质酸/陶瓷磷酸三钙(PHT)功能化的PCL支架中的体外和体内成骨能力。我们的结果表明，这些iPS-MSCs与所测试的两种三维支架在功能上是相容的，并在支架内形成了典型的钙化结构。总之，我们的研究结果表明，通过这种简单的方法获得的iPS-MSCs完全保留了成骨功能，为未来的个性化骨科治疗提供了一种新的解决方案。
We describe a simple method for bone engineering using biodegradable scaffolds with mesenchymal stem cells derived from human induced-pluripotent stem cells (hiPS-MSCs). The hiPS-MSCs expressed mesenchymal markers (CD90, CD73, and CD105), possessed multipotency characterized by tri-lineages differentiation: osteogenic, adipogenic, and chondrogenic, and lost pluripotency – as seen with the loss of markers OCT3/4 and TRA-1-81 – and tumorigenicity. However, these iPS-MSCs are still positive for marker NANOG. We further explored the osteogenic potential of the hiPS-MSCs in synthetic polymer polycaprolactone (PCL) scaffolds or PCL scaffolds functionalized with natural polymer hyaluronan and ceramic TCP (PHT) both in vitro and in vivo. Our results showed that these iPS-MSCs are functionally compatible with the two 3D scaffolds tested and formed typically calcified structure in the scaffolds. Overall, our results suggest the iPS-MSCs derived by this simple method retain fully osteogenic function and provide a new solution towards personalized orthopedic therapy in the future.
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DOI：
10.1126/science.1162494
发表时间：
2008-11-07
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Stadtfeld M;Nagaya M;Utikal J;Weir G;Hochedlinger K
通讯作者：
Hochedlinger K
影响因子：
15.8
作者：
Barberi T;Willis LM;Socci ND;Studer L
通讯作者：
Studer L
影响因子：
14
作者：
Sun, HF;Mei, L;Wang, PY
通讯作者：
Wang, PY
影响因子：
5.2
作者：
Bilousova, Ganna;Jun, Du Hyun;King, Karen B.;De Langhe, Stijn;Chick, Wallace S.;Torchia, Enrique C.;Chow, Kelsey S.;Klemm, Dwight J.;Roop, Dennis R.;Majka, Susan M.
通讯作者：
Majka, Susan M.
影响因子：
6
作者：
Chen, Yen Shun;Pelekanos, Rebecca A.;Fisk, Nicholas M.
通讯作者：
Fisk, Nicholas M.