CCCH-type zinc finger family in maize: genome-wide identification, classification and expression profiling under abscisic acid and drought treatments.
CCCH-type zinc finger family in maize: genome-wide identification, classification and expression profiling under abscisic acid and drought treatments.
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DOI：
10.1371/journal.pone.0040120
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Cheng B
中科院分区：
文献类型：
--
作者：
Peng X;Zhao Y;Cao J;Zhang W;Jiang H;Li X;Ma Q;Zhu S;Cheng B
CCCH型锌指蛋白包含一个大的蛋白质家族。越来越多的证据表明，该家族的成员是RNA结合蛋白，在mRNA加工中具有调节功能。与动物相比，CCCH型锌指蛋白在植物生长发育中的功能还不清楚。在这里，我们进行了玉米（Zea mays L.）CCCH型锌指基因的全基因组调查。通过描述每个家族成员的基因结构、系统发育关系和染色体位置。启动子序列和表达谱的推定应力响应成员进行了研究。共鉴定出68个玉米CCCH基因（ZmC 3 H1 -68），通过系统发育分析将其分为7个类群。这68个基因分布在10条染色体上，有15个片段重复事件，表明片段重复在玉米CCCH家族的扩展中起着重要作用。Ka/Ks比值表明，CCCH家族重复基因在重复事件后主要经历了纯化选择，功能分化有限。12个玉米CCCH基因与其他已知的胁迫响应基因的拟南芥被发现含有推定的胁迫响应顺式元件在其启动子区域。这些基因中的7个被选择用于进一步的定量实时PCR分析，在5个代表性的玉米组织中显示出差异表达模式，并且随着时间的推移，响应于脱落酸和干旱处理。在这项研究中提出的结果提供了玉米CCCH蛋白的基本信息，并形成这些蛋白的未来功能研究的基础，特别是对于那些成员可能在响应非生物胁迫中发挥重要作用。
CCCH-type zinc finger proteins comprise a large protein family. Increasing evidence suggests that members of this family are RNA-binding proteins with regulatory functions in mRNA processing. Compared with those in animals, functions of CCCH-type zinc finger proteins involved in plant growth and development are poorly understood. Here, we performed a genome-wide survey of CCCH-type zinc finger genes in maize (Zea mays L.) by describing the gene structure, phylogenetic relationships and chromosomal location of each family member. Promoter sequences and expression profiles of putative stress-responsive members were also investigated. A total of 68 CCCH genes (ZmC3H1-68) were identified in maize and divided into seven groups by phylogenetic analysis. These 68 genes were found to be unevenly distributed on 10 chromosomes with 15 segmental duplication events, suggesting that segmental duplication played a major role in expansion of the maize CCCH family. The Ka/Ks ratios suggested that the duplicated genes of the CCCH family mainly experienced purifying selection with limited functional divergence after duplication events. Twelve maize CCCH genes grouped with other known stress-responsive genes from Arabidopsis were found to contain putative stress-responsive cis-elements in their promoter regions. Seven of these genes chosen for further quantitative real-time PCR analysis showed differential expression patterns among five representative maize tissues and over time in response to abscisic acid and drought treatments. The results presented in this study provide basic information on maize CCCH proteins and form the foundation for future functional studies of these proteins, especially for those members of which may play important roles in response to abiotic stresses.
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Pfam：氏族、网络工具和服务。
影响因子：
14.9
作者：
Finn, Robert D.;Mistry, Jaina;Schuster-Bockler, Benjamin;Griffiths-Jones, Sam;Hollich, Volker;Lassmann, Timo;Moxon, Simon;Marshall, Mhairi;Khanna, Ajay;Durbin, Richard;Eddy, Sean R.;Sonnhammer, Erik L. L.;Bateman, Alex
通讯作者：
Bateman, Alex
影响因子：
7.4
作者：
Haberer, G;Hindemitt, T;Mayer, KFX
通讯作者：
Mayer, KFX
影响因子：
11.6
作者：
Li, ZS;Thomas, TL
通讯作者：
Thomas, TL
DOI：
10.1083/jcb.200811072
发表时间：
2009-04-20
期刊：
The Journal of cell biology
影响因子：
--
作者：
Hurt JA;Obar RA;Zhai B;Farny NG;Gygi SP;Silver PA
通讯作者：
Silver PA
DOI：
10.1002/prot.340170405
发表时间：
1993-12-01
期刊：
PROTEINS-STRUCTURE FUNCTION AND GENETICS
影响因子：
--
作者：
BORK, P
通讯作者：
BORK, P