Differentially expressed genes in the pre-eclamptic placenta: a systematic review and meta-analysis.
Differentially expressed genes in the pre-eclamptic placenta: a systematic review and meta-analysis.
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DOI：
10.1371/journal.pone.0068991
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Afink GB
中科院分区：
文献类型：
--
作者：
Kleinrouweler CE;van Uitert M;Moerland PD;Ris-Stalpers C;van der Post JA;Afink GB
系统回顾子痫前期患者胎盘组织基因表达数据的文献，分析差异表达基因的元特征，以寻找新的诊断标记物。Medline至2011年2月11日，使用与胎盘、基因表达和基因表达阵列相关的网状术语和关键词；使用术语“Platent*”的GEO数据库；以及符合条件的初步研究的参考列表，无限制。从检索到的1068项研究中，我们纳入了对妊娠晚期胎盘组织进行基因表达阵列分析的原始出版物，以及报告了子痫前期与正常血压对照组差异表达基因的原始出版物。两名评价者独立确定了符合条件的研究，提取了描述性和基因表达数据，并评估了研究质量。使用基于比较元特征分析算法的选票计数方法，我们从独立基因签名集合中确定了表征差异表达基因显著交集的元签名。我们确定了33个符合条件的妊娠晚期胎盘组织基因表达阵列研究，其中包括30个关于mRNA表达的数据集和4个关于microRNA表达的数据集。子痫前期胎盘元签名由40个注释基因转录本和17个microRNAs组成。至少有一半的mRNA转录本编码一种由细胞分泌的蛋白质，可能会作为生物标记物。除了已知和有效的基因外，我们还鉴定了14个以前没有报道的与子痫前期相关的转录本，其中大部分也在妊娠早期胎盘中表达，其中3个编码分泌蛋白。
To systematically review the literature on human gene expression data of placental tissue in pre-eclampsia and to characterize a meta-signature of differentially expressed genes in order to identify novel putative diagnostic markers. Medline through 11 February 2011 using MeSH terms and keywords related to placenta, gene expression and gene expression arrays; GEO database using the term “placent*”; and reference lists of eligible primary studies, without constraints. From 1068 studies retrieved from the search, we included original publications that had performed gene expression array analyses of placental tissue in the third trimester and that reported on differentially expressed genes in pre-eclampsia versus normotensive controls. Two reviewers independently identified eligible studies, extracted descriptive and gene expression data and assessed study quality. Using a vote-counting method based on a comparative meta-profiling algorithm, we determined a meta-signature that characterizes the significant intersection of differentially expressed genes from the collection of independent gene signatures. We identified 33 eligible gene expression array studies of placental tissue in the 3rd trimester comprising 30 datasets on mRNA expression and 4 datasets on microRNA expression. The pre-eclamptic placental meta-signature consisted of 40 annotated gene transcripts and 17 microRNAs. At least half of the mRNA transcripts encode a protein that is secreted from the cell and could potentially serve as a biomarker. In addition to well-known and validated genes, we identified 14 transcripts not reported previously in relation to pre-eclampsia of which the majority is also expressed in the 1st trimester placenta, and three encode a secreted protein.
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