TILLING: a shortcut in functional genomics.
TILLING: a shortcut in functional genomics.
复制标题
耕作：功能基因组学的快捷方式。
DOI：
10.1007/s13353-011-0061-1
复制
发表时间：
2011-11
影响因子：
2.4
通讯作者：
Maluszynski, Miroslaw
中科院分区：
文献类型：
--
作者：
Kurowska, Marzena;Daszkowska-Golec, Agata;Gruszka, Damian;Marzec, Marek;Szurman, Miriam;Szarejko, Iwona;Maluszynski, Miroslaw
关键词：
大规模基因组测序项目的最新进展为传统突变技术在正向和反向遗传学策略中的应用开辟了新的可能性。TILLING（靶向诱导的基因组局部损伤）是十年前开发的，作为插入诱变的替代方法。它利用了经典诱变、序列可用性和高通量筛选靶序列中的核苷酸多态性。TILLING作为反向遗传学策略的主要优点是，它可以应用于任何物种，无论其基因组大小和倍性水平如何。TILLING方案提供了在基因组中随机分布的高频率的点突变。化学试剂产生高比率核苷酸取代的巨大致突变潜力已通过各种植物物种中TILLING群体报告的高密度突变得到证明。对于其中大多数，几个基因的分析揭示了1个突变/200-500 kb的筛选和更高的密度观察到多倍体物种，如小麦。高通量TILLING允许快速且低成本地发现在植物中诱导的新等位基因。几个研究中心为各种植物物种建立了TILLING公共服务。TILLING程序的最新趋势依赖于生物信息学工具的多样化，突变检测的新方法，包括错配特异性和敏感性核酸内切酶，以及使用下一代测序技术进行LI-COR筛选和单核苷酸多态性（SNP）发现的各种替代方案。TILLING策略在功能基因组学中有许多应用。此外，这种吞吐量方法在基础和应用研究中的广泛应用已经通过修改原始TILLING策略（如Ecotilling或Deletion TILLING）来实现。
Recent advances in large-scale genome sequencing projects have opened up new possibilities for the application of conventional mutation techniques in not only forward but also reverse genetics strategies. TILLING (Targeting Induced Local Lesions IN Genomes) was developed a decade ago as an alternative to insertional mutagenesis. It takes advantage of classical mutagenesis, sequence availability and high-throughput screening for nucleotide polymorphisms in a targeted sequence. The main advantage of TILLING as a reverse genetics strategy is that it can be applied to any species, regardless of its genome size and ploidy level. The TILLING protocol provides a high frequency of point mutations distributed randomly in the genome. The great mutagenic potential of chemical agents to generate a high rate of nucleotide substitutions has been proven by the high density of mutations reported for TILLING populations in various plant species. For most of them, the analysis of several genes revealed 1 mutation/200–500 kb screened and much higher densities were observed for polyploid species, such as wheat. High-throughput TILLING permits the rapid and low-cost discovery of new alleles that are induced in plants. Several research centres have established a TILLING public service for various plant species. The recent trends in TILLING procedures rely on the diversification of bioinformatic tools, new methods of mutation detection, including mismatch-specific and sensitive endonucleases, but also various alternatives for LI-COR screening and single nucleotide polymorphism (SNP) discovery using next-generation sequencing technologies. The TILLING strategy has found numerous applications in functional genomics. Additionally, wide applications of this throughput method in basic and applied research have already been implemented through modifications of the original TILLING strategy, such as Ecotilling or Deletion TILLING.
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影响因子：
5.1
作者：
Gady AL;Hermans FW;Van de Wal MH;van Loo EN;Visser RG;Bachem CW
通讯作者：
Bachem CW
方案：一种改进的高通量方法，用于以96孔格式生成植物基因分型的组织样品（ICE-CAP 2.0）。
影响因子：
5.1
作者：
Clark KA;Krysan PJ
通讯作者：
Krysan PJ
影响因子：
5.4
作者：
Feiz, L.;Martin, J. M.;Giroux, M. J.
通讯作者：
Giroux, M. J.
影响因子：
4.4
作者：
Coassin S;Brandstätter A;Kronenberg F
通讯作者：
Kronenberg F
影响因子：
7.4
作者：
Bush, Susan M.;Krysan, Patrick J.
通讯作者：
Krysan, Patrick J.