Stimulation of Posterior Thalamic Nuclei Induces Photophobic Behavior in Mice.
Stimulation of Posterior Thalamic Nuclei Induces Photophobic Behavior in Mice.
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作者：
Sowers LP;Wang M;Rea BJ;Taugher RJ;Kuburas A;Kim Y;Wemmie JA;Walker CS;Hay DL;Russo AF
偏头痛的一个标志是头痛恐惧症。在小鼠中，降钙素基因相关肽（CGRP）是一种已知在偏头痛中起关键作用的神经肽，可诱导小鼠产生类似于偏头痛的行为。在这项研究中，我们试图确定CGRP可以诱导恐惧症的大脑部位。我们专注于后丘脑区域，其中包含对光和硬脑膜刺激都有反应的神经元，并具有CGRP结合位点。我们在野生型小鼠中用光遗传学刺激和急性CGRP注射来探测该区域。由于光厌恶试验在历史上已被用于研究动物的焦虑样反应，我们在光独立的开放领域测定焦虑，并询问是否刺激大脑区域，导水管周围灰质，诱导焦虑会产生类似的结果后丘脑刺激。海马被用作解剖学对照，以确保任何大脑区域的刺激都不会诱导光厌恶行为。在开放视野试验中，丘脑后核神经元细胞体的光遗传激活在明亮和昏暗的光线下都会引起光厌恶，而不会出现焦虑样反应。将CGRP注射到丘脑后区引发了类似的光厌恶行为，但没有焦虑。与丘脑后核相反，背侧导水管周围灰质细胞体的光遗传学刺激引起光厌恶和焦虑样反应，而CGRP注射没有影响。在背侧海马，无论是光刺激，也没有CGRP注射影响光厌恶或开放领域的行为。刺激丘脑后核能够在小鼠中启动光厌恶信号，该信号可能被CGRP调制以引起偏头痛中的光恐惧症。
A hallmark of migraine is photophobia. In mice, photophobia-like behavior is induced by calcitonin gene-related peptide (CGRP), a neuropeptide known to be a key player in migraine. In this study we sought to identify sites within the brain from which CGRP could induce photophobia. We focused on the posterior thalamic region, which contains neurons responsive to both light and dural stimulation and has CGRP binding sites. We probed this area with both optogenetic stimulation and acute CGRP injections in wild-type mice. Since the light aversion assay has historically been used to investigate anxiety-like responses in animals, we measured anxiety in a light-independent open field assay and asked if stimulation of a brain region, the periaqueductal gray, that induces anxiety would yield similar results to posterior thalamic stimulation. The hippocampus was used as an anatomical control to ensure that light-aversive behaviors could not be induced by the stimulation of any brain region. Optogenetic activation of neuronal cell bodies in the posterior thalamic nuclei elicited light aversion in both bright and dim light without an anxiety-like response in an open field assay. Injection of CGRP into the posterior thalamic region triggered similar light-aversive behavior without anxiety. In contrast to the posterior thalamic nuclei, optogenetic stimulation of dorsal periaqueductal gray cell bodies caused both light aversion and an anxiety-like response, while CGRP injection had no effect. In the dorsal hippocampus, neither optical stimulation nor CGRP injection affected light aversion or open field behaviors. Stimulation of posterior thalamic nuclei is able to initiate light-aversive signals in mice that may be modulated by CGRP to cause photophobia in migraine.
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影响因子：
7.4
作者：
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