Fine mapping and epistatic interactions of the vernalization gene VRN-D4 in hexaploid wheat.
Fine mapping and epistatic interactions of the vernalization gene VRN-D4 in hexaploid wheat.
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DOI：
10.1007/s00438-013-0788-y
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发表时间：
2014-02
期刊：
影响因子：
--
通讯作者：
Dubcovsky J
中科院分区：
文献类型：
--
作者：
Kippes N;Zhu J;Chen A;Vanzetti L;Lukaszewski A;Nishida H;Kato K;Dvorak J;Dubcovsky J
小麦春化需求主要受VRN1、VRN2、VRN3和VRN4基因控制。前三个已经被克隆，并且在所有三个基因组中都有同源基因。VRN4仅存在于D基因组(VRN-D4)中，尚未被克隆。我们构建了VRN-D4区域的高密度遗传图谱，并将VRN-D4定位在5D染色体着丝粒区域的0.09 cM间隔内。利用VRN-D4供体三重Dirk F产生的端粒5D染色体，我们确定VRN-D4位于短臂上。VRN-D4候选区域与Brachypodium disachyon 4号染色体上2.24个Mb区域共线，该区域包括127个预测基因。这些基因中有10个预测了在发育中的作用，但我们没有检测到与VRN-D4相关的功能多态。两个重组事件将VRN-D4从拟南芥春化基因VIL1的小麦同源基因Tavil-D1中分离出来，证实该基因不是VRN-D4的候选基因。我们检测到VRN-D4与其他四个控制春化需求的基因(VRN-A1、VRN-B1、VRN-D1和VRN-B3)之间存在显著的相互作用，这证实了VRN-D4是春化途径的一部分，它要么位于VRN1、VRN2和VRN3基因的上游，要么是涉及VRN1、VRN2和VRN3基因的调控反馈环的一部分。VRN-D4基因的精确定位及其与其他春化基因的互作特性为VRN-D4在小麦改良中的利用以及我们目前克隆该春化基因提供了有价值的信息。本文的在线版本(doi：10.1007/s00438-0130788-y)包含补充材料，授权用户可以使用。
Wheat vernalization requirement is mainly controlled by the VRN1, VRN2, VRN3, and VRN4 genes. The first three have been cloned and have homoeologs in all three genomes. VRN4 has been found only in the D genome (VRN-D4) and has not been cloned. We constructed a high-density genetic map of the VRN-D4 region and mapped VRN-D4 within a 0.09 cM interval in the centromeric region of chromosome 5D. Using telocentric 5D chromosomes generated from the VRN-D4 donor Triple Dirk F, we determined that VRN-D4 is located on the short arm. The VRN-D4 candidate region is colinear with a 2.24 Mb region on Brachypodium distachyon chromosome 4, which includes 127 predicted genes. Ten of these genes have predicted roles in development but we detected no functional polymorphisms associated to VRN-D4. Two recombination events separated VRN-D4 from TaVIL-D1, the wheat homolog of Arabidopsis vernalization gene VIL1, confirming that this gene is not a candidate for VRN-D4. We detected significant interactions between VRN-D4 and other four genes controlling vernalization requirement (Vrn-A1, Vrn-B1, Vrn-D1, and Vrn-B3), which confirmed that VRN-D4 is part of the vernalization pathway and that it is either upstream or is part of the regulatory feedback loop involving VRN1, VRN2 and VRN3 genes. The precise mapping of VRN-D4 and the characterization of its interactions with other vernalization genes provide valuable information for the utilization of VRN-D4 in wheat improvement and for our current efforts to clone this vernalization gene. The online version of this article (doi:10.1007/s00438-013-0788-y) contains supplementary material, which is available to authorized users.
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影响因子：
4.4
作者：
Akhunov ED;Akhunova AR;Anderson OD;Anderson JA;Blake N;Clegg MT;Coleman-Derr D;Conley EJ;Crossman CC;Deal KR;Dubcovsky J;Gill BS;Gu YQ;Hadam J;Heo H;Huo N;Lazo GR;Luo MC;Ma YQ;Matthews DE;McGuire PE;Morrell PL;Qualset CO;Renfro J;Tabanao D;Talbert LE;Tian C;Toleno DM;Warburton ML;You FM;Zhang W;Dvorak J
通讯作者：
Dvorak J
影响因子：
3.1
作者：
Fu, Daolin;Dunbar, Mignon;Dubcovsky, Jorge
通讯作者：
Dubcovsky, Jorge
影响因子：
7.4
作者：
Hemming, Megan N.;Peacock, W. James;Trevaskis, Ben
通讯作者：
Trevaskis, Ben
影响因子：
12.3
作者：
Krasileva KV;Buffalo V;Bailey P;Pearce S;Ayling S;Tabbita F;Soria M;Wang S;IWGS Consortium;Akhunov E;Uauy C;Dubcovsky J
通讯作者：
Dubcovsky J
影响因子：
4.5
作者：
Chen A;Dubcovsky J
通讯作者：
Dubcovsky J