Investigating the role of circulating CXCR5-expressing CD8+ T-cells as a biomarker for bacterial infection in subjects with pneumonia
Investigating the role of circulating CXCR5-expressing CD8+ T-cells as a biomarker for bacterial infection in subjects with pneumonia
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研究循环表达 CXCR5 的 CD8 T 细胞作为肺炎受试者细菌感染生物标志物的作用
DOI：
10.1186/s12931-019-1011-4
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发表时间：
2019-03
期刊：
影响因子：
--
通讯作者：
Cheng Chen
中科院分区：
文献类型：
--
作者：
Yu Shen;Qiuxia Qiu;Mengni Jin;Cheng Chen
BackgroundRecently, lymphoid follicle-confined and circulating CD8+ T-cells expressing the C-X-C chemokine receptor type 5 (CXCR5) were described, which was involved in anti-virus immune response. However, the dynamics and role of circulating CXCR5-expressing CD8+ T-cells during bacterial infection is unknown. So, we asked whether CXCR5+ CD8+ T cells were also generated during bacterial infections in lower respiratory tract.MethodsThe clinical data of 65 pneumonia patients were analyzed. The patients were divided into groups as tuberculosis, bronchiectasis and community or hospital acquired pneumonia (CAP, HAP). The sputum/bronchial secretion or bronchoalveolar lavage fluid (BALF) samples were taken for microbiological examination. The procalcitonin (PCT) was used to evaluate disease severity of these groups and compared among patients. We characterized the number and phenotype (PD-1 and CD103) of CXCR5 + CD8+ T cells in the peripheral circulation by flow cytometry in all individuals and analyzed their association with the serum PCT level and disease severity.ResultsPatients were mainly infected withEscherichia coli, Acinetobacter baumannii, Klebsiella pneumonia (K.p),Pseudomonas aeruginosa, andStaphylococcus aureus. Of note is the finding that PCT was weakly correlated with severity of respiratory infections. Furthermore, it was revealed an increase of CXCR5-expressing CD8+ T cells in peripheral blood of un-controlled CAP and progressive HAP compared controlled CAP and HAP, respectively (P< 0.05). Strikingly, the circulating CXCR5-expressing CD8+ T-cells in K.p-infected group was higher than that non-K.p-infected group (P< 0.05). Meanwhile, the ratio of CXCR5 + CD8+/CD8 was positively correlated with PCT level (P< 0.05). In clinic, the determination of CXCR5-expressing CD8+ T-cells showed better results compared to PCT and can be useful for the prediction of exacerbation of CAP or HAP. Phenotypically, CXCR5+ CD8 + T cell expressed comparable level of inhibitory molecules PD-1 and lower CD103 compared to their CXCR5- counterparts.ConclusionThe circulating CXCR5-expressing CD8+ T-cell has diagnostic value for current pneumonia severity, and could act as a biomarker for identifying a bacteria-associated exacerbation. These cells may provide novel insight for the pathogenesis of pneumonia.
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影响因子：
3.2
作者：
Karaiskos I;Giamarellou H
通讯作者：
Giamarellou H
影响因子：
64.8
作者：
Im SJ;Hashimoto M;Gerner MY;Lee J;Kissick HT;Burger MC;Shan Q;Hale JS;Lee J;Nasti TH;Sharpe AH;Freeman GJ;Germain RN;Nakaya HI;Xue HH;Ahmed R
通讯作者：
Ahmed R
影响因子：
32.4
作者：
Sathaliyawala T;Kubota M;Yudanin N;Turner D;Camp P;Thome JJ;Bickham KL;Lerner H;Goldstein M;Sykes M;Kato T;Farber DL
通讯作者：
Farber DL
DOI：
10.1097/qai.0000000000001700
发表时间：
2018-08
期刊：
JAIDS Journal of Acquired Immune Deficiency Syndromes
影响因子：
--
作者：
F. Perdomo-Celis;N. Taborda;M. Rugeles
通讯作者：
F. Perdomo-Celis;N. Taborda;M. Rugeles
影响因子：
11.8
作者：
Liliana Simon;F. Gauvin;D. Amre;Patrick Saint-Louis;J. Lacroix
通讯作者：
Liliana Simon;F. Gauvin;D. Amre;Patrick Saint-Louis;J. Lacroix