Anopheles stephensi Feeding, Flight Behavior, and Infection With Malaria Parasites are Altered by Ingestion of Serotonin.
Anopheles stephensi Feeding, Flight Behavior, and Infection With Malaria Parasites are Altered by Ingestion of Serotonin.
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DOI：
10.3389/fphys.2022.911097
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发表时间：
2022
影响因子：
4
通讯作者：
中科院分区：
文献类型：
--
作者：
大约有34亿人面临疟疾的风险，这是一种由疟原虫感染引起的疾病。寄生虫，由按蚊传播。患有严重恶性疟疾的个体经常表现出循环血液中生物胺水平的变化，包括血清素或5-羟色胺（5-HT）减少，这些变化与疾病病理学有关。在昆虫中，5-HT是一种重要的神经递质，对昆虫的许多行为和生物学功能起作用。在斯氏按蚊中，我们发现5-HT定位于头部，胸部和中肠的神经支配，这表明肠道到大脑的信号传导轴可以支持摄入5-HT对蚊子生物学和行为反应的影响。考虑到与严重疟疾相关的5-HT血液水平的变化以及5-HT在昆虫神经生理学中的关键作用，我们研究了健康水平5-HT（1.5 µ M）或疟疾相关水平5-HT（0.15 µ M）的输入血液对A.史蒂芬西生物学在这些研究中，我们提供了5-HT和监测的繁殖力，寿命，飞行行为，和血液喂养的A。史蒂芬西我们还评估了5-HT摄入对A. stephensi与小鼠疟原虫约氏约氏疟原虫17XNL和人疟原虫恶性疟原虫的感染。我们的数据表明，与严重疟疾相关的5-HT的摄入增加了蚊子的飞行速度和对宿主气味（CO2）的视觉对象的调查。血液中5-HT的摄入水平与严重疟疾相关，也增加了未感染的A.史蒂芬西在感染P.y. yoelii 17XNL，当存在中肠卵囊时，摄食倾向降低，但当存在子孢子时，摄食倾向增加。除此之外，A. stephensi与5-HT相关的严重疟疾增加了感染P.y. yoelii 17XNL与对照相比，而用健康水平的5-HT处理降低了恶性疟原虫的感染成功率。这些蚊子行为和感染成功的变化可以用作操纵媒介蚊子中5-HT信号的基础，以改善对疟原虫传播的控制。
Approximately 3.4 billion people are at risk of malaria, a disease caused by infection with Plasmodium spp. parasites, which are transmitted by Anopheles mosquitoes. Individuals with severe falciparum malaria often exhibit changes in circulating blood levels of biogenic amines, including reduced serotonin or 5-hydroxytryptamine (5-HT), and these changes are associated with disease pathology. In insects, 5-HT functions as an important neurotransmitter for many behaviors and biological functions. In Anopheles stephensi, we show that 5-HT is localized to innervation in the head, thorax, and midgut, suggesting a gut-to-brain signaling axis that could support the effects of ingested 5-HT on mosquito biology and behavioral responses. Given the changes in blood levels of 5-HT associated with severe malaria and the key roles that 5-HT plays in insect neurophysiology, we investigated the impact of ingesting blood with healthy levels of 5-HT (1.5 µM) or malaria-associated levels of 5-HT (0.15 µM) on various aspects of A. stephensi biology. In these studies, we provisioned 5-HT and monitored fecundity, lifespan, flight behavior, and blood feeding of A. stephensi. We also assessed the impact of 5-HT ingestion on infection of A. stephensi with the mouse malaria parasite Plasmodium yoelii yoelii 17XNL and the human malaria parasite Plasmodium falciparum. Our data show that ingestion of 5-HT associated with severe malaria increased mosquito flight velocity and investigation of visual objects in response to host odor (CO2). 5-HT ingestion in blood at levels associated with severe malaria also increased the tendency to take a second blood meal 4 days later in uninfected A. stephensi. In mosquitoes infected with P. y. yoelii 17XNL, feeding tendency was decreased when midgut oocysts were present but increased when sporozoites were present. In addition to these effects, treatment of A. stephensi with 5-HT associated with severe malaria increased infection success with P. y. yoelii 17XNL compared to control, while treatment with healthy levels of 5-HT decreased infection success with P. falciparum. These changes in mosquito behavior and infection success could be used as a basis to manipulate 5-HT signaling in vector mosquitoes for improved control of malaria parasite transmission.
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影响因子：
4.7
作者：
LOO, YH
通讯作者：
LOO, YH
DOI：
10.1016/b978-0-12-805350-8.00002-7
发表时间：
2017-01-01
期刊：
ARTHROPOD VECTOR: CONTROLLER OF DISEASE TRANSMISSION, VOL 1: VECTOR MICROBIOME AND INNATE IMMUNITY OF ARTHROPODS
影响因子：
--
作者：
Luckhart, Shirley;Riehle, Michael A.
通讯作者：
Riehle, Michael A.
影响因子：
3.7
作者：
Huser A;Eschment M;Güllü N;Collins KAN;Böpple K;Pankevych L;Rolsing E;Thum AS
通讯作者：
Thum AS
DOI：
10.1073/pnas.1722379115
发表时间：
2018-05-22
影响因子：
11.1
作者：
Chapman PD;Burkland R;Bradley SP;Houot B;Bullman V;Dacks AM;Daly KC
通讯作者：
Daly KC
DOI：
10.4269/ajtmh.2000.62.284
发表时间：
2000-02-01
影响因子：
3.3
作者：
Dobbie, M;Crawley, J;Surtees, R
通讯作者：
Surtees, R