Anatomy and behavioral function of serotonin receptors in Drosophila melanogaster larvae.
Anatomy and behavioral function of serotonin receptors in Drosophila melanogaster larvae.
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DOI：
10.1371/journal.pone.0181865
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Thum AS
中科院分区：
文献类型：
--
作者：
Huser A;Eschment M;Güllü N;Collins KAN;Böpple K;Pankevych L;Rolsing E;Thum AS
生物胺5-羟色胺（5-HT）是大多数动物门中枢神经系统中的重要神经活性分子。5-HT与特定的G蛋白偶联和配体门控离子受体结合，以调节动物行为的特定方面。在果蝇中，就像在许多其他昆虫中一样，这包括运动和进食的调节。由于其遗传的顺从性和神经元的简单性，果蝇幼虫已经成为一个有用的模型，研究化学感受行为的解剖和分子基础。这对于嗅觉系统尤其如此，嗅觉系统在神经元信息处理的前三个顺序上主要描述到突触水平。在这里，我们集中在果蝇幼虫的5-HT受体系统。在一个由解剖学和行为实验组成的双向方法中，我们描述了单个5-HT受体对幼稚和后天化学感觉行为的分布和影响。我们的数据表明，5-HT 1A，5-HT 1B和5-HT 7是幼稚的嗅觉和味觉选择行为，以及食欲和厌恶的联想嗅觉学习和记忆。相反，我们发现，5-HT/5-HT 2A信号在整个发展，但不是作为一个急性神经元功能，影响联想嗅觉学习和记忆使用高盐浓度作为一个负的非条件刺激。这些发现首次描述了5-HT信号在果蝇幼虫的学习和记忆中的参与。从长远来看，这些结果可能会揭示发育，5-HT依赖的原则有关的强化处理可能与成年果蝇和其他昆虫。
The biogenic amine serotonin (5-HT) is an important neuroactive molecule in the central nervous system of the majority of animal phyla. 5-HT binds to specific G protein-coupled and ligand-gated ion receptors to regulate particular aspects of animal behavior. In Drosophila, as in many other insects this includes the regulation of locomotion and feeding. Due to its genetic amenability and neuronal simplicity the Drosophila larva has turned into a useful model for studying the anatomical and molecular basis of chemosensory behaviors. This is particularly true for the olfactory system, which is mostly described down to the synaptic level over the first three orders of neuronal information processing. Here we focus on the 5-HT receptor system of the Drosophila larva. In a bipartite approach consisting of anatomical and behavioral experiments we describe the distribution and the implications of individual 5-HT receptors on naïve and acquired chemosensory behaviors. Our data suggest that 5-HT1A, 5-HT1B, and 5-HT7 are dispensable for larval naïve olfactory and gustatory choice behaviors as well as for appetitive and aversive associative olfactory learning and memory. In contrast, we show that 5-HT/5-HT2A signaling throughout development, but not as an acute neuronal function, affects associative olfactory learning and memory using high salt concentration as a negative unconditioned stimulus. These findings describe for the first time an involvement of 5-HT signaling in learning and memory in Drosophila larvae. In the longer run these results may uncover developmental, 5-HT dependent principles related to reinforcement processing possibly shared with adult Drosophila and other insects.
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影响因子：
3.7
作者：
Becnel J;Johnson O;Luo J;Nässel DR;Nichols CD
通讯作者：
Nichols CD
影响因子：
2.2
作者：
Blenau, W;Baumann, A
通讯作者：
Baumann, A
影响因子：
2.6
作者：
Colas, JF;Launay, JM;Maroteaux, L
通讯作者：
Maroteaux, L
影响因子：
1.2
作者：
Apostolopoulou, Anthi A.;Widmann, Annekathrin;Thum, Andreas S.
通讯作者：
Thum, Andreas S.
果蝇小分子药物筛选将 5HT2A 受体确定为摄食调节靶点
影响因子：
4.6
作者：
Gasque, Gabriel;Conway, Stephen;Huang, Juan;Rao, Yi;Vosshall, Leslie B
通讯作者：
Vosshall, Leslie B