Local ablation by micro-electric knife
Local ablation by micro-electric knife
复制标题
微型电刀局部消融
DOI：
--
复制
发表时间：
2012
期刊：
影响因子：
--
通讯作者：
F. Arai
中科院分区：
文献类型：
--
作者：
Y. Yamanishi;H. Kuriki;S. Sakuma;K. Onda;F. Arai
我们首次成功利用微型电刀进行卵母细胞去核。控制放电输出功率和导电面积以适应水下和大气压环境下的细胞级烧蚀。通过铜丝周围的玻璃壳绝缘并涂上银浆实现局部烧蚀，防止在水下排放时产生气泡。这种锋利的电刀在刀的末端在线和玻璃尖端之间设计了一个小空间，这有助于稳定放电。通过使用坚固的玻璃壳绝缘材料和从电极内部注入的单个微尺度气泡，与传统线电极相比，消融区域的宽度成功减少了一半以上。此外，Hoechst染色的细胞核也被成功去除，且损伤区域有限，这对于玻璃毛细管的手动操作来说是一项艰巨的任务。这种低成本的微电刀可以在各种环境下制造任何目标材料，有可能为微纳生物工程领域的一种新的制造方法做出贡献。
We have firstly succeeded in enucleation of oocyte by using micro-electric knife. The discharged output power and conductive area were controlled to adapt the cellular-order ablation under water and under atmospheric pressure environment. The local area ablation was obtained by the glass shell insulation around the copper wire with silver paste, and which prevent the bubble generation when it was discharged under the water. This fabricated sharp electric knife has a designed small space at the end of the knife between the wire and the glass tip, and which contribute to stabilize the electric discharge. The width of the ablation region was successfully reduced to more than a half compare to the conventional wire electrode, by using the robust glass shell insulation and a single micro-scale bubble injected from inside of the electrode. Moreover, the nucleus dyed by Hoechst was also removed successfully with limited damage region which is the difficult task for manual operation with glass capillary. This low cost micro-electric knife can fabricate any objective material under various environments and it has a possibility to contribute to a new fabrication method in micro-nano bioengineering field.
用于眼内手术的脉冲电子雪崩刀（PEAK）。
DOI：
--
发表时间：
2001
期刊：
Investigative ophthalmology & visual science.
影响因子：
--
作者：
Palanker,DV;Miller,JM;Marmor,MF;Sanislo,SR;Huie,P;Blumenkranz,MS
通讯作者：
Blumenkranz,MS
影响因子：
6.1
作者：
Zeng, Fei;Rohde, Christopher B.;Yanik, Mehmet Fatih
通讯作者：
Yanik, Mehmet Fatih