A novel electrode-pipette design for simultaneous recording of extracellular spikes and iontophoretic drug application in awake behaving monkeys.
A novel electrode-pipette design for simultaneous recording of extracellular spikes and iontophoretic drug application in awake behaving monkeys.
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DOI：
10.1016/j.jneumeth.2006.05.032
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发表时间：
2006-12-15
影响因子：
3
通讯作者：
Gieselmann, M. A.
中科院分区：
文献类型：
--
作者：
Thiele, A.;Delicato, L. S.;Roberts, M. J.;Gieselmann, M. A.
我们开发了一种新颖的电极-移液管组合（EPC）设计，可以在不需要引导管或在电极插入之前机械打开硬脑膜的情况下进入清醒行为的灵长类动物的大脑结构。EPC包括一个蚀刻钨在玻璃电极两侧的两个移液管，允许局部和高度控制的离子渗透管理的神经活性物质。这些内皮细胞具有优异的单细胞分离特性，并且足够坚固，可以在开颅手术或硬脑膜刮伤（即即使在纤维瘢痕组织大量形成之后）后穿透灵长类动物硬脑膜长达8周。我们发现EPCs可以在被动注视和执行注意力要求高的任务时选择性地操纵灵长类V1的胆碱能系统。
We developed a novel design of an electrode–pipette combination (EPC) which allows access to brain structures in awake behaving primates without the need for guide tubes or to mechanically open the dura prior to electrode insertion. The EPC consists of an etched tungsten in glass electrode flanked by two pipettes which allow for local and highly controlled iontophoretic administration of neuroactive substances. These EPCs have excellent single cell isolation properties and are sturdy enough to penetrate the primate dura for up to 8 weeks following either a craniotomy or a dura scrape (i.e. even after substantial built up of fibrous scar tissue). We show that the EPCs can be used to selectively manipulate the cholinergic system in primate V1 during passive fixation and while animals perform an attentionally demanding task.
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影响因子：
2.5
作者：
Davidson, MC;Marrocco, RT
通讯作者：
Marrocco, RT
影响因子：
2.5
作者：
Hasselmo, ME;Linster, C;Cekic, M
通讯作者：
Cekic, M
影响因子：
2.5
作者：
Patil, MM;Linster, C;Hasselmo, ME
通讯作者：
Hasselmo, ME
影响因子：
2.5
作者：
SATO, H;HATA, Y;TSUMOTO, T
通讯作者：
TSUMOTO, T
影响因子：
3.4
作者：
Witte, EA;Marrocco, RT
通讯作者：
Marrocco, RT