Expression of the calcium-activated potassium channel in upper and lower segment human myometrium during pregnancy and parturition.
Expression of the calcium-activated potassium channel in upper and lower segment human myometrium during pregnancy and parturition.
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DOI：
10.1186/1477-7827-7-27
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发表时间：
2009-04-05
期刊：
影响因子：
--
通讯作者：
Ni X
中科院分区：
文献类型：
--
作者：
Gao L;Cong B;Zhang L;Ni X
大电导钙激活钾通道（BKCa）在妊娠期子宫收缩的调控中起重要作用。从子宫静止到子宫收缩活动增强的变化可能与子宫肌层内BKCa的时空表达有关。本研究的目的是检测BKCa α-和β-亚基在子宫上段（US）和下段（LS）区域的表达，并研究这些蛋白在从三种功能状态获得的US和LS子宫肌层中的可能差异表达：（1）非妊娠（NP）;（2）足月妊娠未分娩（TNL）和（3）足月妊娠分娩（TL）。从子宫切除术的非妊娠女性和择期剖腹产或紧急剖腹产的妊娠女性中收集子宫肌层活检。分别用Western blot和免疫组化方法检测BKCa α和β亚基在US和LS子宫肌层中的蛋白表达水平和细胞定位。BKCa α和β亚基主要定位于非妊娠和妊娠患者的US和LS子宫肌层中的子宫肌层平滑肌。BKCa α-亚单位的水平在美国，但不是在LS是显着高于在妊娠期间获得的子宫肌层中测量的NP子宫肌层。在US和LS中，TL中BKCa α亚基的表达低于TNL活检。TL组US和LS肌层β-亚基的表达均较TNL组显著降低。NP组与TNL组BKCa β亚基表达无显著性差异。我们的研究结果表明，表达的BKCa α-和β-亚基在妊娠子宫肌层减少在劳动，这是一致的，在分娩开始时的子宫肌层活动。
Large conductance calcium-activated potassium channel (BKCa) plays an important role in the control of uterine contractility during pregnancy. The change from uterine quiescence to enhanced contractile activity may be associated with the spatial and temporal expression of BKCa within myometrium. The objectives of this study were to examine the expression of BKCa alpha- and beta-subunit in upper segment (US) and lower segment (LS) regions of uterus, and to investigate for the possibly differential expression of these proteins in US and LS myometrium obtained from three functional states: (1) non-pregnant (NP); (2) term pregnant not in labour (TNL) and (3) term pregnant in labour (TL). Myometrial biopsies were collected from non-pregnant women at hysterectomy and pregnant women at either elective caesarean section or emergency caesarean section. Protein expression level and cellular localization of BKCa alpha- and beta-subunit in US and LS myometrium were determined by Western blot analysis and immunohistochemistry, respectively. BKCa alpha- and beta-subunit were predominantly localized to myometrial smooth muscle in both US and LS myometrium obtained from non-pregnant and pregnant patients. The level of BKCa alpha-subunit in US but not in LS was significantly higher in NP myometrium than those measured in myometrium obtained during pregnancy. Lower expression of BKCa alpha-subunit in both US and LS was found in TL than in TNL biopsies. Expression of beta-subunit in both US and LS myometrium was significantly reduced in TL group compared with those measured in TNL group. There was no significant difference in BKCa beta-subunit expression in either US or LS between NP and TNL group. Our results suggest that expression of BKCa alpha- and beta-subunit in pregnant myometrium is reduced during labour, which is consistent with the myometrial activity at the onset of parturition.
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DOI：
10.1098/rspb.1993.0002
发表时间：
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影响因子：
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