Preparation and evaluation of human choroid extracellular matrix scaffolds for the study of cell replacement strategies.
Preparation and evaluation of human choroid extracellular matrix scaffolds for the study of cell replacement strategies.
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DOI：
10.1016/j.actbio.2017.05.011
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发表时间：
2017-07-15
影响因子：
9.7
通讯作者：
Mullins RF
中科院分区：
文献类型：
--
作者：
Chirco KR;Worthington KS;Flamme-Wiese MJ;Riker MJ;Andrade JD;Ueberheide BM;Stone EM;Tucker BA;Mullins RF
脉络膜毛细血管的内皮细胞（EC）是年龄相关性黄斑变性（AMD）期间最先丢失的细胞类型之一，并且细胞替代疗法目前是晚期AMD患者非常有希望的选择。我们试图开发一种可靠的方法来生产人脉络膜细胞外基质（ECM）支架，这将允许脉络膜EC（CEC）的替代策略的研究在一个环境中，非常相似的天然组织。依次用Triton X-100、SDS和DNA酶处理人RPE/脉络膜组织以去除所有天然细胞。虽然所有的细胞被成功地从组织中去除，胶原蛋白IV，弹性蛋白，和层粘连蛋白仍然存在，与保存的无细胞血管管的架构。然后将ECM支架与外源性EC共培养，以确定组织是否可以支持细胞生长并允许EC重新整合到脱细胞脉络膜脉管系统中。猴和人的EC都在脱细胞组织的脉络膜毛细血管管中定居。总之，这些数据表明，我们的脱细胞方法足以去除所有细胞物质，但又足够温和，以保留组织结构，并允许优化细胞替代策略。
Endothelial cells (ECs) of the choriocapillaris are one of the first cell types lost during age-related macular degeneration (AMD), and cell replacement therapy is currently a very promising option for patients with advanced AMD. We sought to develop a reliable method for the production of human choroidal extracellular matrix (ECM) scaffolds, which will allow for the study of choroidal EC (CEC) replacement strategies in an environment that closely resembles the native tissue. Human RPE/choroid tissue was treated sequentially with Triton X-100, SDS, and DNase to remove all native cells. While all cells were successfully removed from the tissue, collagen IV, elastin, and laminin remained, with preserved architecture of the acellular vascular tubes. The ECM scaffolds were then co-cultured with exogenous ECs to determine if the tissue can support cell growth and allow EC reintegration into the decellularized choroidal vasculature. Both monkey and human ECs took up residence in the choriocapillary tubes of the decellularized tissue. Together, these data suggest that our decellularization methods are sufficient to remove all cellular material yet gentle enough to preserve tissue structure and allow for the optimization of cell replacement strategies.
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影响因子：
14
作者：
De Waele, Jorrit;Reekmans, Kristien;Ponsaerts, Peter
通讯作者：
Ponsaerts, Peter
影响因子：
82.9
作者：
Ott, Harald C.;Clippinger, Ben;Vacanti, Joseph P.
通讯作者：
Vacanti, Joseph P.
影响因子：
20.1
作者：
Thum, Thomas;Hoeber, Sarah;Bauersachs, Johann
通讯作者：
Bauersachs, Johann
影响因子：
14
作者：
Luo, Hailang;Lu, Yongbo;Jin, Yan
通讯作者：
Jin, Yan
影响因子：
6.2
作者：
ABIHANNA, D;WAKEFIELD, D;WATKINS, S
通讯作者：
WATKINS, S