Differential Leukocyte and Platelet Profiles in Distinct Models of Traumatic Brain Injury.
Differential Leukocyte and Platelet Profiles in Distinct Models of Traumatic Brain Injury.
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DOI：
10.3390/cells10030500
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发表时间：
2021-02-26
期刊：
影响因子：
6
通讯作者：
Sullivan PG
中科院分区：
文献类型：
--
作者：
Hubbard WB;Banerjee M;Vekaria H;Prakhya KS;Joshi S;Wang QJ;Saatman KE;Whiteheart SW;Sullivan PG
在美国，每年有300多万人受到创伤性脑损伤的影响。人们越来越认识到，创伤性脑损伤可以产生全身性的改变，这种改变在一定程度上是通过血脑屏障(BBB)功能障碍和血脑细胞相互作用来传播的。因此，血小板和白细胞参与了脑外伤后血栓性炎症的机制。虽然这些机制已经在挫伤脑损伤的实验模型中进行了研究，但对于轻度闭合性脑损伤后的急性变化却知之甚少。为了研究脑外伤后血小板动力学和生物能量学的作用，我们在小鼠中采用了两种不同的、公认的脑外伤模型：受控皮质撞击(CCI)挫伤脑损伤模型和闭合性脑损伤(CHI)轻度弥漫性脑损伤模型。伤后进行血液学参数、血小板-中性粒细胞聚集和血小板呼吸测定。CCI致伤后早期血白细胞计数下降，CHI致伤后早期血白细胞计数升高。与假手术组相比，CCI后血小板-中性粒细胞聚集明显改变。此外，血小板生物能偶联效率在CCI后6h有一过性降低，在24h有所增加。与假手术组相比，CHI后6h，完整血小板氧化磷酸化水平降低，而在24h，氧化磷酸化水平升高。综上所述，这些数据表明，脑创伤引发了血小板-白细胞动力学和血小板代谢的变化，这可能是时间和损伤相关的，为血小板携带脑损伤的外周特征提供了证据。两种不同类型的脑外伤后，血小板生物能量学的独特趋势提示了其在预后中的应用潜力。
Traumatic brain injury (TBI) affects over 3 million individuals every year in the U.S. There is growing appreciation that TBI can produce systemic modifications, which are in part propagated through blood–brain barrier (BBB) dysfunction and blood–brain cell interactions. As such, platelets and leukocytes contribute to mechanisms of thromboinflammation after TBI. While these mechanisms have been investigated in experimental models of contusion brain injury, less is known regarding acute alterations following mild closed head injury. To investigate the role of platelet dynamics and bioenergetics after TBI, we employed two distinct, well-established models of TBI in mice: the controlled cortical impact (CCI) model of contusion brain injury and the closed head injury (CHI) model of mild diffuse brain injury. Hematology parameters, platelet-neutrophil aggregation, and platelet respirometry were assessed acutely after injury. CCI resulted in an early drop in blood leukocyte counts, while CHI increased blood leukocyte counts early after injury. Platelet-neutrophil aggregation was altered acutely after CCI compared to sham. Furthermore, platelet bioenergetic coupling efficiency was transiently reduced at 6 h and increased at 24 h post-CCI. After CHI, oxidative phosphorylation in intact platelets was reduced at 6 h and increased at 24 h compared to sham. Taken together, these data demonstrate that brain trauma initiates alterations in platelet-leukocyte dynamics and platelet metabolism, which may be time- and injury-dependent, providing evidence that platelets carry a peripheral signature of brain injury. The unique trend of platelet bioenergetics after two distinct types of TBI suggests the potential for utilization in prognosis.
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影响因子：
4.6
作者：
Hubbard, W. Brad;Harwood, Christopher L.;Sullivan, Patrick G.
通讯作者：
Sullivan, Patrick G.
DOI：
10.1097/nen.0000000000000115
发表时间：
2014-10
影响因子：
3.2
作者：
Bolton AN;Saatman KE
通讯作者：
Saatman KE
影响因子：
1.9
作者：
Guillotte, Andrew R.;Herbert, Joseph P.;Litofsky, N. Scott
通讯作者：
Litofsky, N. Scott
影响因子：
6.9
作者：
Chodobski A;Zink BJ;Szmydynger-Chodobska J
通讯作者：
Szmydynger-Chodobska J
影响因子：
16.6
作者：
Ehinger JK;Piel S;Ford R;Karlsson M;Sjövall F;Frostner EÅ;Morota S;Taylor RW;Turnbull DM;Cornell C;Moss SJ;Metzsch C;Hansson MJ;Fliri H;Elmér E
通讯作者：
Elmér E