The cyclic pattern of blood alcohol levels during continuous ethanol feeding in rats: the effect of feeding S-adenosylmethionine.
The cyclic pattern of blood alcohol levels during continuous ethanol feeding in rats: the effect of feeding S-adenosylmethionine.
复制标题
DOI：
10.1016/j.yexmp.2010.03.004
复制
发表时间：
2010-06
影响因子：
3.6
通讯作者：
French, S. W.
中科院分区：
文献类型：
--
作者：
Bardag-Gorce, F.;Li, J.;Oliva, J.;Lu, S. C.;French, B. A.;French, S. W.
关键词：
为改变乙醇对大鼠肝脏的影响，将DNA和组蛋白甲基化的主要甲基供体S-腺苷甲硫氨酸（SAMe）用乙醇喂养一个月。研究了以下参数以确定SAMe的影响;肝组织学、血液酒精循环（BAL）、从微阵列分析中挖掘的基因表达的变化、组蛋白甲基化的变化、肝SAMe水平及其代谢物和ADH的变化。SAMe改变了脂肪肝的类型，降低了肝脏ALT水平，并阻止了胃内乙醇喂养引起的BAL循环。微阵列分析表明，SAMe喂养防止乙醇喂养诱导的基因表达的变化，大概是通过诱导H3 K27 me 3和基因沉默。H3 K27 me 3在有或没有乙醇进料的情况下通过SAMe显著增加。它的结论是，SAMe喂养稳定的全球基因表达，使血液酒精循环中的基因表达的变化被阻止。
S-adenosylmethionine (SAMe), the major methyl donor for DNA and histone methylation was fed with ethanol for one month in order to modify the effects of ethanol on rat liver. The following parameters were studied to determine the effects of SAMe; liver histology, the blood alcohol cycle (BAL), changes in gene expression mined from microarray analysis, changes in histone methylation, changes in liver SAMe levels and its metabolites and ADH. SAMe changed the type of fatty liver, reduced liver ALT levels and prevented the BAL cycle caused by intragastric ethanol feeding. Microarray analysis showed that SAMe feeding prevented most of the changes in gene expression induced by ethanol feeding, presumably by inducing H3K27me3 and gene silencing. H3K27me3 was significantly increased by SAMe with or without ethanol feeding. It is concluded that SAMe feeding stabilized global gene expression so that the changes in gene expression involved in the blood alcohol cycle were prevented.
登录
查看更多内容
DOI：
10.1002/hep.22470
发表时间：
2008-10
期刊：
Hepatology (Baltimore, Md.)
影响因子：
--
作者：
Hritz I;Mandrekar P;Velayudham A;Catalano D;Dolganiuc A;Kodys K;Kurt-Jones E;Szabo G
通讯作者：
Szabo G
影响因子：
6.1
作者：
Li, J;French, BA;French, SW
通讯作者：
French, SW
影响因子：
4.3
作者：
Oliva, Joan;Dedes, Jennifer;Bardag-Gorce, Fawzia
通讯作者：
Bardag-Gorce, Fawzia
影响因子：
13.5
作者：
Huang, ZZ;Mato, JM;Lu, SC
通讯作者：
Lu, SC
DOI：
10.1152/ajpgi.2000.279.1.g118
发表时间：
2000-07-01
影响因子：
4.5
作者：
Li, J;Nguyen, V;French, SW
通讯作者：
French, SW