Transepithelial migration of Toxoplasma gondii is linked to parasite motility and virulence.
Transepithelial migration of Toxoplasma gondii is linked to parasite motility and virulence.
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DOI：
10.1084/jem.20020258
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发表时间：
2002-06-17
期刊：
影响因子：
--
通讯作者：
Sibley LD
中科院分区：
文献类型：
--
作者：
Barragan A;Sibley LD
口服后，刚地弓形虫穿过肠上皮，扩散到深部组织，并穿过生物屏障，如胎盘和血脑屏障，到达引起严重病理的部位。为了研究这些过程的细胞基础，我们利用极化宿主细胞单层和细胞外基质在体外研究了弓形虫的迁移。迁移需要活跃的寄生虫运动，高毒力的I型菌株始终表现出比非毒力的II型和III型菌株更强的迁移能力。I型菌株寄生虫也表现出更大的体外跨肠转运能力，并直接渗透到固有层和血管内皮。一个强毒型I型寄生虫亚群在体外表现出长距离迁移（LDM）表型，而非强毒型II和III型菌株不表达这种表型。通过克隆表达LDM表型的寄生虫，使其在体内和体外的迁移能力显著提高。弓形虫迁移能力的上调可能在建立新感染和慢性感染重新激活后的传播中发挥重要作用。
After oral ingestion, Toxoplasma gondii crosses the intestinal epithelium, disseminates into the deep tissues, and traverses biological barriers such as the placenta and the blood-brain barrier to reach sites where it causes severe pathology. To examine the cellular basis of these processes, migration of T. gondii was studied in vitro using polarized host cell monolayers and extracellular matrix. Transmigration required active parasite motility and the highly virulent type I strains consistently exhibited a superior migratory capacity than the nonvirulent type II and type III strains. Type I strain parasites also demonstrated a greater capacity for transmigration across mouse intestine ex vivo, and directly penetrated into the lamina propria and vascular endothelium. A subpopulation of virulent type I parasites exhibited a long distance migration (LDM) phenotype in vitro, that was not expressed by nonvirulent type II and type III strains. Cloning of parasites expressing the LDM phenotype resulted in substantial increase of migratory capacity in vitro and in vivo. The potential to up-regulate migratory capacity in T. gondii likely plays an important role in establishing new infections and in dissemination upon reactivation of chronic infections.
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影响因子：
2.4
作者：
MEIS, JFGM;VERHAVE, JP;MEUWISSEN, JHET
通讯作者：
MEUWISSEN, JHET
影响因子：
3.1
作者：
Channon, JY;Seguin, RM;Kasper, LH
通讯作者：
Kasper, LH
影响因子：
4.4
作者：
Mordue, DG;Monroy, F;Sibley, LD
通讯作者：
Sibley, LD
影响因子：
56.9
作者：
JOINER, KA;FUHRMAN, SA;MELLMAN, I
通讯作者：
MELLMAN, I
影响因子：
4.4
作者：
Del Rio, L;Bennouna, S;Denkers, EY
通讯作者：
Denkers, EY