Development of luminescent pH sensor films for monitoring bacterial growth through tissue.
Development of luminescent pH sensor films for monitoring bacterial growth through tissue.
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DOI：
10.1002/adhm.201300101
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发表时间：
2014-02
影响因子：
10
通讯作者：
Anker, Jeffrey N.
中科院分区：
文献类型：
--
作者：
Wang, Fenglin;Raval, Yash;Chen, Hongyu;Tzeng, Tzuen-Rong J.;DesJardins, John D.;Anker, Jeffrey N.
虽然植入式医疗器械(IMD)有许多好处，但它们容易受到细菌定植和感染。这类感染很难治疗，因为细菌可能会在植入物表面形成生物膜，这会降低抗生素的渗透率，并产生低pH值和低氧气的局部休眠区域。此外，这些感染很难及早发现，因为生物膜往往局限在表面。在此，开发了一种光学传感器薄膜来检测植入表面的局部酸中毒。该薄膜既包含作为光源的上转换粒子(UCP)，也包含改变发光光谱的pH指示剂。当用980 nm的光照射时，UCPs会产生深度穿透的红光发射，同时在组织中产生可以忽略不计的自发荧光。基本形式的pH指示剂在661 nm处比在671 nm处吸收更多的上转换发光，因此光谱比指示pH。将这种pH传感器膜植入6-7 mm厚的猪组织下不会对校准曲线产生实质性影响，因为峰间距很近。此外，表皮葡萄球菌在传感器表面的生长会导致局部pH值下降，这可以通过组织非侵入性地检测到。
Although implanted medical devices (IMDs) offer many benefits, they are susceptible to bacterial colonization and infections. Such infections are difficult to treat because bacteria could form biofilms on the implant surface, which reduce antibiotics penetration and generate local dormant regions with low pH and low oxygen. In addition, these infections are hard to detect early because biofilms are often localized on the surface. Herein, an optical sensor film is developed to detect local acidosis on an implanted surface. The film contains both upconverting particles (UCPs) that serve as a light source and a pH indicator that alters the luminescence spectrum. When irradiated with 980 nm light, the UCPs produce deeply penetrating red light emission, while generating negligible autofluorescence in the tissue. The basic form of the pH indicator absorbs more of upconversion luminescence at 661 nm than at 671 nm and consequently the spectral ratio indicates pH. Implanting this pH sensor film beneath 6-7 mm of porcine tissue does not substantially affect the calibration curve because the peaks are closely spaced. Furthermore, growth of Staphylococcus epidermidis on the sensor surface causes a local pH decrease that can be detected non-invasively through the tissue.
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影响因子：
4.1
作者：
Kirkland, JJ;Henderson, JW;Claessens, HA
通讯作者：
Claessens, HA
影响因子：
7.4
作者：
Mader, Heike S.;Wolfbeis, Otto S.
通讯作者：
Wolfbeis, Otto S.
影响因子：
3.8
作者：
Salthouse C;Hildebrand S;Weissleder R;Mahmood U
通讯作者：
Mahmood U
DOI：
10.1039/c3an00093a
发表时间：
2013-06-07
期刊：
The Analyst
影响因子：
--
作者：
Ray A;Yoon HK;Koo Lee YE;Kopelman R;Wang X
通讯作者：
Wang X
影响因子：
6.2
作者：
Konttinen, YT;Takagi, M;Santavirta, S
通讯作者：
Santavirta, S