The amnesiac gene is involved in the regulation of thermal nociception in Drosophila melanogaster.
The amnesiac gene is involved in the regulation of thermal nociception in Drosophila melanogaster.
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DOI：
10.3109/01677060903419751
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发表时间：
2010-03
影响因子：
1.9
通讯作者：
Kitamoto T
中科院分区：
文献类型：
--
作者：
Aldrich BT;Kasuya J;Faron M;Ishimoto H;Kitamoto T
伤害感受是动物避免能够引起严重组织损伤的有害刺激的能力的基本机制。已经确定，在果蝇Drosophila melanogaster中，由无痛基因（pain）编码的瞬时受体电位（TRP）通道是检测热和机械伤害性刺激所必需的。然而，对果蝇中控制伤害性行为的其他遗传成分知之甚少。遗忘基因（amn）编码一种假定的神经肽前体，对稳定嗅觉记忆很重要，并参与其他联想和非联想学习的各个方面。先前的研究表明，amn也调节乙醇敏感性和睡眠。在这里，我们表明，amn在伤害性感受中起着额外的关键作用。我们的数据表明，amn突变幼虫和成虫对有害热刺激（> ~ 40 °C）的反应明显低于野生型。amn突变体在热伤害感受中的表型与疼痛突变体的表型非常相似，在表达amn RNAi的果蝇中进行表型复制，并且这种表型通过野生型amn转基因的表达而被拯救。我们的研究结果提供了令人信服的证据，amn是一种新的遗传成分的机制，调节果蝇的热伤害感受。
Nociception is a mechanism fundamental to the ability of animals to avoid noxious stimuli capable of causing serious tissue damage. It has been established that in the fruit fly Drosophila melanogaster, the transient receptor potential (TRP) channel encoded by the painless gene (pain) is required for detecting thermal and mechanical noxious stimuli. Little is known, however, about other genetic components that control nociceptive behaviors in Drosophila. The amnesiac gene (amn), which encodes a putative neuropeptide precursor, is important for stabilizing olfactory memory, and is involved in various aspects of other associative and non-associative learning. Previous studies have indicated that amn also regulates ethanol sensitivity and sleep. Here we show that amn plays an additional critical role in nociception. Our data show that amn mutant larvae and adults are significantly less responsive to noxious heat stimuli (> ~ 40 °C) than their wild-type counterparts. The phenotype of amn mutants in thermal nociception, which closely resembles that of pain mutants, was phenocopied in flies expressing amn RNAi, and this phenotype was rescued by the expression of a wild-type amn transgene. Our results provide compelling evidence that amn is a novel genetic component of the mechanism that regulates thermal nociception in Drosophila.
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