Identification of Tmem10 as a novel late-stage oligodendrocytes marker for detecting hypomyelination.
Identification of Tmem10 as a novel late-stage oligodendrocytes marker for detecting hypomyelination.
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DOI：
10.7150/ijbs.7526
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发表时间：
2013
影响因子：
9.2
通讯作者：
Chen M
中科院分区：
文献类型：
--
作者：
Jiang W;Yang W;Yang W;Zhang J;Pang D;Gan L;Luo L;Fan Y;Liu Y;Chen M
少突胶质细胞包裹轴突，形成致密的绝缘多层结构，称为髓鞘。Tmem10是一种新型的I型跨膜糖蛋白，在少突胶质细胞中高度表达，其生物学功能在很大程度上仍然未知。此外，Tmem10的表达模式仍然存在一些争议。由于其表达模式的不一致性和缺乏有效的特异性抗体，Tmem10没有被广泛接受为成熟少突胶质细胞的标志物。作为解决这些问题的一种手段，并帮助少突胶质细胞相关疾病的未来研究，我们已经产生了高度特异性的Tmem10抗体。使用这种Tmem10抗体，我们阐明了Tmem10蛋白在出生后2周时首先在小鼠大脑中表达，并随年龄增加，仅在中枢神经系统（CNS）中表达。我们还揭示了Tmem10在晚期少突胶质细胞中特异性表达，并且晚于晚期髓鞘标记物MAG。最后，我们发现Tmem10与MOG和MBP阳性髓鞘纤维共表达，并且在髓鞘形成不足的小鼠模型中显著减少。总之，我们的研究表明，Tmem10可以用作髓鞘形成少突胶质细胞的特异性标志物，并可能用于评价髓鞘形成疾病，如多发性硬化症。
Oligodendrocytes ensheath axons to form compact insulating multilamellar structures known as myelin. Tmem10 is a novel type I transmembrane glycoprotein that is highly expressed in oligodendrocytes and whose biological function remains largely unknown. Furthermore, the expression pattern of Tmem10 remains a matter of some controversy. Given the inconsistency of its expression pattern and the lack of validated specific antibodies, Tmem10 is not widely accepted as a marker for mature oligodendrocytes. As a means to solve these problems and to aid future studies of oligodendrocyte-associated diseases, we have generated a highly specific Tmem10 antibody. Using this Tmem10 antibody, we clarify that Tmem10 protein is firstly expressed at 2 weeks in the postnatal mouse brain with age-related increase, only in the central nervous system (CNS). We also reveal that Tmem10 is expressed specifically in late stage oligodendrocytes and later than MAG, a late-stage myelin marker. Finally, we show that Tmem10 co-expresses with MOG- and MBP-positive myelin fibers and is dramatically reduced in a hypomyelination mouse model. In conclusion, our study demonstrates that Tmem10 can be used as a specific marker for myelinating oligodendrocytes and perhaps for the evaluation of myelination diseases, such as multiple sclerosis.
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影响因子：
30.8
作者：
Lappe-Siefke, C;Goebbels, S;Nave, KA
通讯作者：
Nave, KA
DOI：
10.1016/s0378-4347(99)00524-1
发表时间：
2000-01-14
期刊：
JOURNAL OF CHROMATOGRAPHY B
影响因子：
--
作者：
Gibert, S;Bakalara, N;Santarelli, X
通讯作者：
Santarelli, X
影响因子：
9.2
作者：
Long, X;Lin, Y;Avruch, J
通讯作者：
Avruch, J
影响因子：
2.4
作者：
Kippert, Angelika;Trajkovic, Katarina;Simons, Mikael
通讯作者：
Simons, Mikael
影响因子：
15.9
作者：
Delarasse, C;Daubas, P;Pham-Dinh, D
通讯作者：
Pham-Dinh, D