Doxycycline-loaded nanotube-modified adhesives inhibit MMP in a dose-dependent fashion.
Doxycycline-loaded nanotube-modified adhesives inhibit MMP in a dose-dependent fashion.
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DOI：
10.1007/s00784-017-2215-y
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发表时间：
2018-04
影响因子：
3.4
通讯作者：
Bottino MC
中科院分区：
文献类型：
--
作者：
Palasuk J;Windsor LJ;Platt JA;Lvov Y;Geraldeli S;Bottino MC
本文评估了负载 DOX 的纳米管改性粘合剂释放的多西环素 (DOX) 的药物负载量、释放动力学和基质金属蛋白酶 (MMP) 抑制作用。 DOX 被选为模型药物，因为它是美国食品和药物管理局批准的唯一 MMP 抑制剂。使用不同浓度的 DOX 溶液将药物装载到纳米管中。 DOX 浓度的增加显着提高了 DOX 的负载量。改性粘合剂是通过将负载 DOX 的纳米管掺入商业产品的粘合剂树脂中制成的。研究了改性粘合剂的转化度 (DC)、努氏显微硬度、DOX 释放动力学、抗菌性、细胞相容性和抗 MMP 活性。加入 DOX 纳米管不会影响 DC、努普显微硬度或细胞兼容性。较高浓度的 DOX 导致改性粘合剂中 DOX 释放量以浓度依赖性方式增加。改性粘合剂释放的 DOX 不会抑制龋齿相关细菌的生长，但更重要的是它确实抑制了 MMP-1 活性。将 DOX 负载到纳米管改性粘合剂中不会损害粘合剂的理化性质，并且释放的 DOX 水平能够抑制 MMP 活性而不产生细胞毒性。从纳米管改性粘合剂中释放的多西环素以浓度依赖性方式抑制 MMP 活性。因此，所提出的纳米管改性粘合剂可能具有作为保持树脂/牙本质粘合稳定性的策略的临床潜力。
This article evaluated the drug loading, release kinetics, and matrix metalloproteinase (MMP) inhibition of doxycycline (DOX) released from DOX-loaded nanotube-modified adhesives. DOX was chosen as the model drug, since it is the only MMP inhibitor approved by the U.S. Food and Drug Administration. Drug loading into the nanotubes was accomplished using DOX solution at distinct concentrations. Increased concentrations of DOX significantly improved the amount of loaded DOX. The modified adhesives were fabricated by incorporating DOX-loaded nanotubes into the adhesive resin of a commercial product. The degree of conversion (DC), Knoop microhardness, DOX release kinetics, antimicrobial, cytocompatibility, and anti-MMP activity of the modified adhesives were investigated. Incorporation of DOX-loaded nanotubes did not compromise DC, Knoop microhardness, or cell compatibility. Higher concentrations of DOX led to an increase in DOX release in a concentration-dependent manner from the modified adhesives. DOX released from the modified adhesives did not inhibit the growth of caries-related bacteria, but more importantly it did inhibit MMP-1 activity. The loading of DOX into the nanotube-modified adhesives did not compromise the physicochemical properties of the adhesives and the released levels of DOX were able to inhibit MMP activity without cytotoxicity. Doxycycline released from the nanotube-modified adhesives inhibited MMP activity in a concentration-dependent fashion. Therefore, the proposed nanotube-modified adhesive may hold clinical potential as a strategy to preserve resin/dentin bond stability.
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影响因子：
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