Tau Protein Binding Modes in Alzheimer's Disease for Cationic Luminescent Ligands.
Tau Protein Binding Modes in Alzheimer's Disease for Cationic Luminescent Ligands.
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DOI：
10.1021/acs.jpcb.1c06019
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发表时间：
2021-10-28
期刊：
影响因子：
--
通讯作者：
Norman P
中科院分区：
文献类型：
--
作者：
Todarwal Y;Gustafsson C;Thi Minh NN;Ertzgaard I;Klingstedt T;Ghetti B;Vidal R;König C;Lindgren M;Nilsson KPR;Linares M;Norman P
The bi-thiophene-vinylene-benzothiazole (bTVBT4) ligand developed for Alzheimer’s disease (AD)-specific detection of amyloid tau has been studied by a combination of several theoretical methods and experimental spectroscopies. With reference to the cryo-EM tau structure of the tau protofilament (, , ), a periodic model system of the fibril was created, and the interactions between this fibril and bTVBT4 were studied with nonbiased molecular dynamics simulations. Several binding sites and binding modes were identified and analyzed, and the results for the most prevailing fibril site and ligand modes are presented. A key validation of the simulation work is provided by the favorable comparison of the theoretical and experimental absorption spectra of bTVBT4 in solution and bound to the protein. It is conclusively shown that the ligand–protein binding occurs at the hydrophobic pocket defined by the residues Ile360, Thr361, and His362. This binding site is not accessible in the Pick’s disease (PiD) fold, and fluorescence imaging of bTVBT4-stained brain tissue samples from patients diagnosed with AD and PiD provides strong support for the proposed tau binding site.
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影响因子：
3.2
作者：
Klingstedt T;Aslund A;Simon RA;Johansson LB;Mason JJ;Nyström S;Hammarström P;Nilsson KP
通讯作者：
Nilsson KP
影响因子：
48
作者：
Sigurdson, Christina J.;Peter, K.;Aguzzi, Adriano
通讯作者：
Aguzzi, Adriano
影响因子：
12.7
作者：
Shi Y;Murzin AG;Falcon B;Epstein A;Machin J;Tempest P;Newell KL;Vidal R;Garringer HJ;Sahara N;Higuchi M;Ghetti B;Jang MK;Scheres SHW;Goedert M
通讯作者：
Goedert M
影响因子：
62.1
作者：
Nguyen PH;Ramamoorthy A;Sahoo BR;Zheng J;Faller P;Straub JE;Dominguez L;Shea JE;Dokholyan NV;De Simone A;Ma B;Nussinov R;Najafi S;Ngo ST;Loquet A;Chiricotto M;Ganguly P;McCarty J;Li MS;Hall C;Wang Y;Miller Y;Melchionna S;Habenstein B;Timr S;Chen J;Hnath B;Strodel B;Kayed R;Lesné S;Wei G;Sterpone F;Doig AJ;Derreumaux P
通讯作者：
Derreumaux P
影响因子：
2.9
作者：
Sjoqvist, Jonas;Maria, Jerome;Lindgren, Mikael
通讯作者：
Lindgren, Mikael