Global analysis of transcriptional regulators in Staphylococcus aureus.
Global analysis of transcriptional regulators in Staphylococcus aureus.
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DOI：
10.1186/1471-2164-14-126
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发表时间：
2013-02-26
期刊：
影响因子：
4.4
通讯作者：
Shaw LN
中科院分区：
文献类型：
--
作者：
Ibarra JA;Pérez-Rueda E;Carroll RK;Shaw LN
金黄色葡萄球菌是一种广泛分布的人类病原体，能够感染宿主的几乎每个生态位。因此，它是导致许多不同疾病的原因。S.金黄色葡萄球菌具有大量的毒力决定因子，其表达由复杂的调控网络调节，其中转录因子（TF）是主要元件。在这项工作中，使用多样性序列分析，我们评估了在社区相关的耐甲氧西林沙门氏菌的TF和σ因子的库。金黄色葡萄球菌（CA-MRSA）菌株USA 300-FPR 3757。共135个TF和西格玛因素被确定和归类为36个调节家族。迄今为止，其中约43%已被实验表征，这表明仍有大量工作要做，以解开这种重要病原体的调控网络。比较了S.金黄色葡萄球菌对1209个测序的细菌基因组进行了鉴定，使我们能够鉴定葡萄球菌科的一组核心的正向转录因子，也使我们能够将潜在的功能分配给以前未表征的转录因子。最后，将USA 300 TF与其他11个S.金黄色葡萄球菌菌株包括：纽曼、COL、JH 1、JH 9、MW 2、Mu 3、Mu 50、N315、RF 122、MRSA 252和MSSA 476。我们确定这些菌株之间的保守的TF，并提出可能的监管相互作用。本文提出的分析强调了S.金黄色葡萄球菌菌株，确定关键的保守的TF之间的葡萄球菌，并提供了独特的见解几个尚未表征的TF。
Staphylococcus aureus is a widely distributed human pathogen capable of infecting almost every ecological niche of the host. As a result, it is responsible for causing many different diseases. S. aureus has a vast array of virulence determinants whose expression is modulated by an intricate regulatory network, where transcriptional factors (TFs) are the primary elements. In this work, using diverse sequence analysis, we evaluated the repertoire of TFs and sigma factors in the community-associated methicillin resistant S. aureus (CA-MRSA) strain USA300-FPR3757. A total of 135 TFs and sigma factors were identified and classified into 36 regulatory families. From these around 43% have been experimentally characterized to date, which demonstrates the significant work still at hand to unravel the regulatory network in place for this important pathogen. A comparison of the TF repertoire of S. aureus against 1209 sequenced bacterial genomes was carried out allowing us to identify a core set of orthologous TFs for the Staphylococacceae, and also allowing us to assign potential functions to previously uncharacterized TFs. Finally, the USA300 TFs were compared to those in eleven other S. aureus strains including: Newman, COL, JH1, JH9, MW2, Mu3, Mu50, N315, RF122, MRSA252 and MSSA476. We identify conserved TFs among these strains and suggest possible regulatory interactions. The analysis presented herein highlights the complexity of regulatory networks in S. aureus strains, identifies key conserved TFs among the Staphylococacceae, and offers unique insights into several as yet uncharacterized TFs.
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影响因子：
2.8
作者：
Colmer-Hamood, Jane A.;Aramaki, Hironori;Hamood, Abdul N.
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Hamood, Abdul N.
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