Characterization of a versatile glycoside hydrolase Cel5M from Pectobacterium carotovorum HG-49 for ramie degumming
Characterization of a versatile glycoside hydrolase Cel5M from Pectobacterium carotovorum HG-49 for ramie degumming
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来自胡萝卜果杆菌 HG-49 的多功能糖苷水解酶 Cel5M 用于苎麻脱胶的表征
DOI：
10.1177/0040517519894748
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发表时间：
2020-01
影响因子：
2.3
通讯作者：
Yu Longjiang
中科院分区：
文献类型：
--
作者：
Wang Yawei;Bai Yun;Shu Tong;Fan Pei;Zhang Huashan;Turunen Ossi;Xiong Hairong;Yu Longjiang
从胡萝卜果胶杆菌中分离鉴定了一种新型多功能糖苷水解酶Cel 5 M，并将其用于苎麻脱胶。在大肠杆菌中表达了含有Cel 5 MD催化结构域及其碳水化合物结合模块（CBM 3）的全长Cel 5 M（GenBank：MH 544226）。杆菌Cel 5 M和Cel 5 MD都显示出切割木薯葡甘露聚糖（KGM）、羧甲基纤维素（CMC）和燕麦斯佩耳特木聚糖的宽底物谱。Cel 5 MD与CMC和KGM的最适温度为55 ℃，而Cel 5 M与KGM的最适温度为65 ℃，与CMC的最适温度为60 ℃。改进的热活性与KGM可能也反映在低得多的Km与KGM比与CMC，表明更强的结合的全长酶与KGM。这两种酶在pH 5.0至9.0的宽pH范围内与KGM高度稳定。与仅使用菌株HG-49应用于苎麻脱胶相比，Cel 5 M酶与野生型胡萝卜黄单胞菌HG-49菌株一起使用显示出显著的改进。此外，整个胡萝卜腐霉Cel 5 M基因在胡萝卜腐霉HG-49中过表达，以提高苎麻脱胶能力。总之，这项研究表明，在原位生产的加工酶可用于纺织工业。
A novel versatile glycoside hydrolase Cel5M was identified from Pectobacterium carotovorum and used in ramie degumming. The full-length Cel5M (GenBank: MH544226) containing the catalytic domain Cel5MD and its carbohydrate-binding module (CBM3) were expressed in E. coli. Both Cel5M and Cel5MD showed a broad substrate spectrum cleaving konjac glucomannan (KGM), carboxymethylcellulose (CMC) and oat spelt xylan. The optimal temperature of Cel5MD was 55oC with CMC and KGM, whereas the optimum temperature of Cel5M was 65oC with KGM and 60oC with CMC. The improved thermal activity with KGM was probably reflected also in the much lower K m with KGM than with CMC, indicating stronger binding of the full-length enzyme with KGM. Both enzymes were highly stable over a broad pH range from pH 5.0 to 9.0 with KGM. The use of the Cel5M enzyme together with the wild-type P. carotovorum HG-49 strain showed significant improvement when compared to using the mere strain HG-49 for application in ramie degumming. Moreover, the whole P. carotovorum Cel5M gene was over-expressed in P. carotovorum HG-49 itself to improve the ramie-degumming capacity. Altogether, this study indicates that in situ production of processing enzymes could be utilized in the textile industry.
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DOI：
10.1007/978-981-287-742-0
发表时间：
2016
期刊：
Handbook of Sustainable Luxury Textiles and Fashion
影响因子：
--
作者：
Miguel Angel Gardetti;S. Muthu
通讯作者：
Miguel Angel Gardetti;S. Muthu
影响因子：
2.3
作者：
Zhengfan Li;Zhaoling Li;R. Ding;Chongwen Yu
通讯作者：
Zhengfan Li;Zhaoling Li;R. Ding;Chongwen Yu
影响因子：
2.7
作者：
Cheng, Yi;Liu, Zhengchu;Wang, Ruijun
通讯作者：
Wang, Ruijun
影响因子：
5.7
作者：
Carla Oliveira;G. Sepúlveda;Tatiana Q. Aguiar;F. M. Gama;L. Domingues
通讯作者：
Carla Oliveira;G. Sepúlveda;Tatiana Q. Aguiar;F. M. Gama;L. Domingues
影响因子：
4.8
作者：
McGregor, Nicholas;Morar, Mariya;Brumer, Harry
通讯作者：
Brumer, Harry