Bacterial Swarmers Enriched During Intestinal Stress Ameliorate Damage.
Bacterial Swarmers Enriched During Intestinal Stress Ameliorate Damage.
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DOI：
10.1053/j.gastro.2021.03.017
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发表时间：
2021-07
期刊：
影响因子：
29.4
通讯作者：
Mani S
中科院分区：
文献类型：
--
作者：
De A;Chen W;Li H;Wright JR;Lamendella R;Lukin DJ;Szymczak WA;Sun K;Kelly L;Ghosh S;Kearns DB;He Z;Jobin C;Luo X;Byju A;Chatterjee S;San Yeoh B;Vijay-Kumar M;Tang JX;Prajapati M;Bartnikas TB;Mani S
细菌群集，表面上的集体运动，很少与人类病理生理学相关。本研究旨在确定细菌群在改善肠道应激中的作用。我们开发了一种多微生物平板琼脂测定法来检测群集，并筛选了具有肠道应激和炎症的小鼠和人类。从化学诱导的小鼠结肠炎以及患有炎症性肠病的人类中，我们开发了分离占主导地位的swamers的技术。我们开发了群体缺陷，但生长和游泳能力的突变体细菌作为同基因对照。我们在患有结肠炎的小鼠中进行了细菌再接种研究、粪便16 S和元转录组学分析，以及体外微生物相互作用研究。我们发现，细菌群是高度预测肠道应激小鼠和人类。我们从小鼠粪便中分离到一种新的肠杆菌群集菌株SM 3。与其突变株相反，SM 3和其他已知的大肠杆菌swarmers消除了小鼠的肠道炎症。用SM 3而不是其突变体治疗结肠炎小鼠，富集了属于拟杆菌目S24-7家族的有益粪便厌氧菌。我们在体内粪便元转录组中观察到SM 3群集相关途径。在体外生长的S24-7富集在SM 3或其突变体的存在下，然而，由于SM 3，而不是突变体，诱导S24-7在体内，我们得出结论，群集在传播SM 3在体内起着至关重要的作用。总的来说，我们的工作确定了一个新的但违反直觉的范例，其中肠道压力允许成群的细菌出现;然而，这些细菌可以治愈肠道炎症。细菌群是应激肠的特征，并且当以高丰度存在时，通过其独特的运动形式促进以微生物组依赖性方式保护免受肠道炎症。
Bacterial swarming, a collective movement on a surface, has rarely been associated with human pathophysiology. This study aims to define a role for bacterial swarmers in amelioration of intestinal stress. We developed a polymicrobial plate agar assay to detect swarming and screened mice and humans with intestinal stress and inflammation. From chemically induced colitis in mice, as well as humans with inflammatory bowel disease, we developed techniques to isolate the dominant swamers. We developed swarm-deficient but growth and swim-competent mutant bacteria as isogenic controls. We performed bacterial re-inoculation studies in mice with colitis, fecal 16S, and meta-transcriptomic analyses, as well as in vitro microbial interaction studies. We show that bacterial swarmers are highly predictive of intestinal stress in mice and humans. We isolated a novel Enterobacter swarming strain, SM3, from mouse feces. SM3 and other known commensal swarmers, in contrast to their mutant strains, abrogated intestinal inflammation in mice. Treatment of colitic mice with SM3, but not its mutants, enriched beneficial fecal anaerobes belonging to the family of Bacteroidales S24–7. We observed SM3 swarming associated pathways in the in vivo fecal meta-transcriptomes. In vitro growth of S24–7 was enriched in presence of SM3 or its mutants; however, since SM3, but not mutants, induce S24–7 in vivo, we conclude that swarming plays an essential role in disseminating SM3 in vivo. Overall, our work identifies a new but counterintuitive paradigm in which intestinal stress allows for the emergence of swarming bacteria; however, these bacteria act to heal intestinal inflammation. Bacterial swarmers are a feature of a stressed intestine, and when present in high abundance protect from intestinal inflammation in a microbiome dependent manner, facilitated by its distinct form of motility.
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DOI：
10.1038/nrgastro.2010.39
发表时间：
2010-05
期刊：
Nature reviews. Gastroenterology & hepatology
影响因子：
--
作者：
通讯作者：
--
影响因子：
11
作者：
McCafferty, Jonathan;Muehlbauer, Marcus;Fodor, Anthony A.
通讯作者：
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影响因子：
2.8
作者：
Barak, Jeri D.;Gorski, Lisa;Narm, Koh-Eun
通讯作者：
Narm, Koh-Eun
DOI：
10.3791/52338
发表时间：
2015-04-07
期刊：
Journal of visualized experiments : JoVE
影响因子：
--
作者：
Morales-Soto N;Anyan ME;Mattingly AE;Madukoma CS;Harvey CW;Alber M;Déziel E;Kearns DB;Shrout JD
通讯作者：
Shrout JD
影响因子：
6.4
作者：
Finkelshtein A;Roth D;Ben Jacob E;Ingham CJ
通讯作者：
Ingham CJ