Preparation and Evaluation of Long-Circulating Deoxypodophyllotoxin-Loaded Liposomes Using Poly(Ethylene Glycol)-Distearoylphosphatidylethanolamine
Preparation and Evaluation of Long-Circulating Deoxypodophyllotoxin-Loaded Liposomes Using Poly(Ethylene Glycol)-Distearoylphosphatidylethanolamine
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聚乙二醇-二硬脂酰磷脂酰乙醇胺长循环负载脱氧鬼臼毒素脂质体的制备与评价
DOI：
10.1007/s12247-016-9246-9
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发表时间：
2016-03
影响因子：
2.6
通讯作者：
Yang Ding
中科院分区：
文献类型：
--
作者：
Shu Wang;Weiqin Wang;Huixia Lv;Jianping Zhou;Lingjia Shen;Lifang Yin;Yang Ding
PurposeDeoxypodophyllotoxin (DPT) is a new potential anti-tumor drug with nearly no water solubility and low permeability. Hence, we prepared a poly (ethylene glycol)-distearoylphosphatidylethanolamine (PEG-DSPE) modified long-circulation liposomes for enhanced solubility and anti-tumor capacity of DPT with clinical expectation.Method and ResultDPT-loaded long-circulation liposomes (DPT-LCLPs) were prepared by thin-film dispersion method, and characteristics including particle size, zeta potential and entrapment efficiency of re-dissolution after lyophilization were 110.5 ± 4.5 nm, −15.06 ± 1.14 mV, and 92.45 ± 5.21 %, respectively. TEM images showed that DPT-LCLPs appeared as spherical or ellipsoidal in shape with multilayer membrane. Moreover, sensitive liquid chromatography method was developed for quantification of DPT concentration in rat plasma with diazepam as internal standard (IS), and the results revealed that two-compartment intravenous model analysis was better with respect to data fitted by Kinetica 4.4 program. The terminal phase half-life (T1/2β) of DPT-LCLPs group was estimated to be approximately 155 min; AUC0−τ was more than three times higher than that of control group, demonstrating a prolonged circulation time due to PEG modification. ConclusionPEG-modified liposomes highly improved the solubility and blood circulation of DPT with simple preparation for potentially enhanced anti-cancer therapy.
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影响因子：
2.6
作者：
Wen, Yi;Meng, Wilson S.
通讯作者：
Meng, Wilson S.
影响因子：
10.8
作者：
Zhang, LF;Granick, S
通讯作者：
Granick, S
影响因子：
5.8
作者：
Yuta Yoshizawa;Y. Kono;K. Ogawara;Toshikiro Kimura;K. Higaki
通讯作者：
Yuta Yoshizawa;Y. Kono;K. Ogawara;Toshikiro Kimura;K. Higaki
影响因子：
11.2
作者：
A. Gabizon;R. Catane;B. Uziely;B. Kaufman;T. Safra;R. Cohen;F. Martín;A. Huang;Y. Barenholz
通讯作者：
A. Gabizon;R. Catane;B. Uziely;B. Kaufman;T. Safra;R. Cohen;F. Martín;A. Huang;Y. Barenholz
影响因子：
4.9
作者：
Sun C;Shen WC;Tu J;Zaro JL
通讯作者：
Zaro JL