Molecular Hydrogen Attenuated N-methyl-N-Nitrosourea Induced Corneal Endothelial Injury by Upregulating Anti-Apoptotic Pathway.
Molecular Hydrogen Attenuated N-methyl-N-Nitrosourea Induced Corneal Endothelial Injury by Upregulating Anti-Apoptotic Pathway.
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分子氢通过上调抗凋亡途径减轻 N-甲基-N-亚硝基脲诱发的角膜内皮损伤
DOI：
10.1167/iovs.62.9.2
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发表时间：
2021-07-01
影响因子：
4.4
通讯作者：
Wang L
中科院分区：
文献类型：
--
作者：
Li R;Qu Y;Li X;Tao Y;Yang Q;Wang J;Diao Y;Li Q;Fang Y;Huang Y;Wang L
Purpose Previous work by our group has demonstrated the value of N-methyl-N-nitrosourea (MNU)-induced corneal endothelial decompensation in animal models. The aim of this study was to investigate the effect of molecular hydrogen (H2) on MNU-induced corneal endothelial cell (CEC) injury and the underlying mechanism. Methods MNU-induced animal models of CEC injury were washed with hydrogen-rich saline (HRS) for 14 days. Immunofluorescence staining, immunohistochemical staining, and corneal endothelial assessment were applied to determine architectural and cellular changes on the corneal endothelium following HRS treatment. MNU-induced cell models of CEC injury were co-cultured with H2. The effect of H2 was examined using morphological and functional assays. Results It was shown that MNU could inhibit the proliferation and specific physiological functions of CECs by increasing apoptosis and decreasing the expression of ZO-1 and Na+/K+-ATPase, whereas H2 improved the proliferation and physiological function of CECs by anti-apoptosis. Cell experiments further confirmed that H2 could reverse MNU damage to CECs by decreasing oxidative stress injury, interfering with the NF-κB/NLRP3 pathway and the FOXO3a/p53/p21 pathway. Conclusions This study suggests that topical application of H2 could protect CECs against corneal damage factors through anti-apoptotic effect, reduce the incidence and severity of corneal endothelial decompensation, and maintain corneal transparency.
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DOI：
10.1080/13510002.2017.1416773
发表时间：
2018-12
期刊：
Redox report : communications in free radical research
影响因子：
--
作者：
Beyfuss K;Hood DA
通讯作者：
Hood DA
影响因子：
0.8
作者：
Gupta, Kishan;Deng, Sophie X.
通讯作者：
Deng, Sophie X.
影响因子：
30.5
作者：
通讯作者：
--
影响因子：
--
作者：
Cejka C;Kossl J;Hermankova B;Holan V;Cejkova J
通讯作者：
Cejkova J
影响因子：
3.4
作者：
Huang, Yang;Ma, Tianju;Li, Zhaohui
通讯作者：
Li, Zhaohui