Combination cisplatin and sulforaphane treatment reduces proliferation, invasion, and tumor formation in epidermal squamous cell carcinoma.
Combination cisplatin and sulforaphane treatment reduces proliferation, invasion, and tumor formation in epidermal squamous cell carcinoma.
复制标题
DOI：
10.1002/mc.22714
复制
发表时间：
2018-01
影响因子：
4.6
通讯作者：
Eckert RL
中科院分区：
文献类型：
--
作者：
Kerr C;Adhikary G;Grun D;George N;Eckert RL
表皮鳞状细胞癌是一种非常常见的癌症类型。早期肿瘤可以通过手术成功治疗，但复发性疾病是侵袭性的，对治疗有抵抗力。顺铂常用作治疗，但结果很少令人满意。因此，需要新的战略。萝卜硫素是一种饮食衍生的癌症预防剂，可有效抑制皮肤癌动物模型中的肿瘤生长。我们监测莱菔硫烷和顺铂作为鳞状细胞癌联合治疗的疗效。两种药物均抑制细胞增殖、癌症干细胞球体的生长、SCC-13和HaCaT细胞的基质胶侵袭和迁移，并且联合治疗更有效。此外，SCC-13细胞衍生的癌症干细胞比非干细胞癌细胞对这些试剂更敏感。两种药物均抑制肿瘤形成，但联合治疗可观察到抑制作用增强。此外，这两种药物都减少了肿瘤驻留癌症干细胞的数量。SFN处理培养的细胞或肿瘤增加细胞凋亡和p21 Cip 1水平，并且两种药剂都增加肿瘤细胞凋亡。我们认为萝卜硫素和顺铂联合治疗能有效抑制肿瘤形成，可能是晚期表皮鳞状细胞癌的一种治疗选择。
Epidermal squamous cell carcinoma is an extremely common type of cancer. Early tumors can be successfully treated by surgery, but recurrent disease is aggressive and resistant to therapy. Cisplatin is often used as a treatment, but the outcome is rarely satisfactory. For this reason new strategies are required. Sulforaphane is a diet-derived cancer prevention agent that is effective in suppressing tumor growth in animal models of skin cancer. We monitored the efficacy of sulforaphane and cisplatin as a combined therapy for squamous cell carcinoma. Both agents suppress cell proliferation, growth of cancer stem cell spheroids, matrigel invasion and migration of SCC-13 and HaCaT cells and combination treatment is more efficient. In addition, SCC-13 cell derived cancer stem cells are more responsive to these agents than non-stem cancer cells. Both agents suppress tumor formation, but enhanced suppression is observed with combined treatment. Moreover, both agents reduce the number of tumor-resident cancer stem cells. SFN treatment of cultured cells or tumors increases apoptosis and p21Cip1 level, and both agents increase tumor apoptosis. We suggest that combined therapy with sulforaphane and cisplatin is efficient in suppressing tumor formation and may be a treatment option for advanced epidermal squamous cell carcinoma.
登录
查看更多内容
影响因子：
11.2
作者：
Fisher ML;Kerr C;Adhikary G;Grun D;Xu W;Keillor JW;Eckert RL
通讯作者：
Eckert RL
影响因子：
8
作者：
Grun D;Adhikary G;Eckert RL
通讯作者：
Eckert RL
DOI：
10.1016/s0190-9622(08)81509-5
发表时间：
1994-05-01
影响因子：
13.8
作者：
MILLER, DL;WEINSTOCK, MA
通讯作者：
WEINSTOCK, MA
影响因子：
3
作者：
Eckert, Richard L.;Adhikary, Gautam;Balasubramanian, Sivaprakasam;Rorke, Ellen A.;Vemuri, Mohan C.;Boucher, Shayne E.;Bickenbach, Jackie R.;Kerr, Candace
通讯作者：
Kerr, Candace
影响因子：
4.7
作者：
Balasubramanian, Sivaprakasam;Adhikary, Gautam;Eckert, Richard L.
通讯作者：
Eckert, Richard L.