Complete sequences of mitochondria genomes of Aedes aegypti and Culex quinquefasciatus and comparative analysis of mitochondrial DNA fragments inserted in the nuclear genomes.
Complete sequences of mitochondria genomes of Aedes aegypti and Culex quinquefasciatus and comparative analysis of mitochondrial DNA fragments inserted in the nuclear genomes.
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DOI：
10.1016/j.ibmb.2011.05.006
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发表时间：
2011-10
影响因子：
3.8
通讯作者：
Severson, David W.
中科院分区：
文献类型：
--
作者：
Behura, Susanta K.;Lobo, Neil F.;Haas, Brian;deBruyn, Becky;Lovin, Diane D.;Shumway, Martin F.;Puiu, Daniela;Romero-Severson, Jeanne;Nene, Vishvanath;Severson, David W.
我们给出了两种重要蚊子的线粒体基因组的完整序列，埃及伊蚊和致倦库蚊分别是登革热病毒和淋巴丝虫病的主要媒介。埃及按蚊线粒体基因组全长16,655个核苷酸，致倦库蚊线粒体基因组长15,587个核苷酸，但均含有13个蛋白质编码基因、22个转移RNA(TRNA)基因、1个12S核糖体RNA(RRNA)基因、1个16S rRNA基因和一个控制区(CR)。基因组大小的差异是由于控制区长度的差异。我们还以比较的方式分析了两种蚊子之间线粒体DNA类似序列(NUMTs)的核拷贝插入。该基因序列约占埃及按蚊基因组的0.008%，占致倦库蚊基因组的0.001%。在两个基因组中预测的蛋白质编码基因的内含子中都发现了几个NUMT(埃及伊蚊32个基因，而致倦库蚊只有4个基因)。这些含有NUMT的基因在这两个物种之间没有同源基因，也没有在同样含有NUMT的基因组中有相似的拷贝。进一步观察到，含有NUMT的基因相对较长，但GC含量低于非NUMT的平行拷贝。此外，在基因和非基因NUMT之间存在着广泛的同源性，这可能在这些基因组中NUMT的基因组重排中发挥重要作用。我们的研究为理解核mtDNA序列在这些蚊子基因组复杂性中的作用提供了新的见解。
We present complete sequences of the mitochondrial genomes for two important mosquitoes, Aedes aegypti and Culex quinquefasciatus, that are major vectors of dengue virus and lymphatic filariasis, respectively. The A. aegypti mitochondrial genome is 16,655 bp in length and that of Culex quinquefasciatus is 15,587 bp, yet both contain 13 protein coding genes, 22 transfer RNA (tRNA) genes, one 12S ribosomal RNA (rRNA) gene, one 16S rRNA gene and a control region (CR) in the same order. The difference in the genome size is due to the difference in the length of the control region. We also analyzed insertions of nuclear copies of mtDNA-like sequences (NUMTs) in a comparative manner between the two mosquitoes. The NUMT sequences occupy ~0.008% of the A. aegypti genome and ~ 0.001% of the C. quinquefasciatus genome. Several NUMTs were found localized in the introns of predicted protein coding genes in both genomes (32 genes in A. aegypti but only four in C. quinquefasciatus). None of these NUMT-containing genes had an ortholog between the two species or had paralogous copies within a genome that was also NUMT-containing. It was further observed that the NUMT-containing genes were relatively longer but had lower GC content compared to the NUMT-less paralogous copies. Moreover, stretches of homologies are present among the genic and non-genic NUMTs that may play important roles in genomic rearrangement of NUMTs in these genomes. Our study provides new insights on understanding the roles of nuclear mtDNA sequences in genome complexities of these mosquitoes.
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影响因子：
4.5
作者：
Hazkani-Covo E;Covo S
通讯作者：
Covo S
影响因子：
4.4
作者：
Lascaro D;Castellana S;Gasparre G;Romeo G;Saccone C;Attimonelli M
通讯作者：
Attimonelli M
影响因子：
3.9
作者：
Podnar, Martina;Haring, Elisabeth;Mayer, Werner
通讯作者：
Mayer, Werner
影响因子：
10.7
作者：
Pons, J;Vogler, AP
通讯作者：
Vogler, AP
影响因子：
2.6
作者：
Black, W. C.;Bernhardt, S. A.
通讯作者：
Bernhardt, S. A.