Measurement of Reflexive Feeding Response in Drosophila.
Measurement of Reflexive Feeding Response in Drosophila.
复制标题
果蝇反射性进食反应的测量。
DOI：
10.1101/pdb.prot108092
复制
发表时间：
2023
影响因子：
--
通讯作者：
Keene,AlexC
中科院分区：
文献类型：
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作者：
Brown,Elizabeth;Worden,Kurtresha;Li,Yuanyuan;Masek,Pavel;Keene,AlexC
区分有益营养的食物来源和有潜在危险的食物来源的能力对生物体的生存至关重要。在这里，我们描述了一个味觉试验，测量反射性喂养反应对一个给定的味道。为了检查对可溶性化合物的味觉偏好，将一种开胃味剂应用于长鼻，并记录长鼻延伸的比例。这种单蝇试验可以应用于任何遗传背景的成年果蝇和设施，检查神经回路和编码反射性味觉反应的分子过程。此外，该分析需要很少的定制组件，因此可以很容易地建立在实验室与最少的专业知识，研究苍蝇的行为。
The ability to distinguish between food sources that are good and provide nutrients and those that are potentially dangerous is crucial to the survival of an organism. Here, we describe a taste assay that measures the reflexive feeding response to a given tastant. To examine taste preference for a soluble compound, an appetitive tastant is applied to the proboscis, and the proportion of proboscis extensions are recorded. This single-fly assay may be applied to adult Drosophila of any genetic background and facilities examination of the neural circuitry and molecular processes encoding the reflexive taste response. Furthermore, this assay requires few custom components and therefore can be easily established in laboratories with minimal expertise in the study of fly behavior.
DOI：
10.3791/193
发表时间：
2007-01-01
期刊：
Journal of visualized experiments : JoVE
影响因子：
--
作者：
Shiraiwa, Takashi;Carlson, John R
通讯作者：
Carlson, John R